Related Experiment Video
Updated: Dec 23, 2025
Biological Compatibility Profile on Biomaterials for Bone Regeneration
Published on: November 16, 2018
The effect of trace elements on BMP-2, BMP-7 and STRO-1+ cells in hip replacement
Xiaodong Fu1, Weili Wang1, Xiaomiao Li1
1Department of Orthopedics, School of Medicine, South Campus, Renji Hospital, Shanghai Jiaotong University, Shanghai, China.
Insights
Trace elements in the proximal femur correlate with bone morphogenetic protein 7 (BMP-7) and STRO-1+ cells, suggesting a link to prosthesis loosening. This study provides an experimental basis for future treatments.
Area of Science:
- Orthopedic Surgery
- Biochemistry
- Biomaterials Science
Background:
- Prosthesis loosening is a significant complication in hip replacement surgery.
- Understanding the biological factors influencing implant stability is crucial for improving patient outcomes.
Purpose of the Study:
- To investigate the relationship between trace elements in the proximal femur, bone morphogenetic proteins (BMP-2, BMP-7), and STRO-1+ cells.
- To analyze the potential role of these factors in hip prosthesis loosening.
Main Methods:
- Cultured bone marrow mesenchymal stem cells (BMSCs) from hip replacement patients.
- Analyzed STRO-1+ cells via flow cytometry.
- Measured BMP-2 and BMP-7 expression using ELISA.
- Quantified trace elements in supernatant via radioimmunoassay.
- Correlated findings with bone mineral density (BMD).
Main Results:
- Trace element correlations varied by separation area and were independent of BMP-2.
- Calcium (Ca2+), Magnesium (Mg2+), and Vitamin D3 (VD3) showed correlations with BMP-7 and STRO-1+ cells.
- Positive correlations were observed between VD3, parathyroid hormone (PTH), zinc, and BMD in specific bone regions.
Conclusions:
- Trace elements in the proximal femur may be associated with hip prosthesis loosening.
- Findings provide an experimental foundation for developing targeted therapies for prosthesis loosening.
Abstract:
To explore the correlation between the trace elements in the proximal femur and BMP-2, BMP-7 and STRO-1+ cells in hip replacement, and analyze the therapeutic effect of prosthesis loosening in clinic. Fifty-one patients undergone the first hip replacement in xxx hospital from August 2016 to August 2019 were selected as the study subjects, including 26 females and 25 males, aged 52-89 years. The bone marrow mesenchymal stem cells (BMSCs) were cultured in vitro for flow cytometry, and the string-1+ in BMSCs was detected and analyzed. After that, the expression of bone morphogenetic protein 2 (BMP-2) and bone morphogenetic protein 7 (BMP-7) in the cells were detected by enzyme-linked immunosorbent assay, the content of trace elements in the supernatant was detected by radioimmunoassay, and the collected data were analyzed statistically. In the analysis of the content of trace elements, it was found that the correlation between trace elements was dependent on the separation area, and all trace elements had no correlation with BMP2. Ca2+, Mg2+ were correlated with the level of BMP7 and Ca2+, VD3 was correlated with the percentage of STOR-1+ cells. Further analysis showed that the correlation between trace elements was dependent on bone mineral density (BMD) area, and there was a positive correlation between vitamin D3 (VD3), parathyroid hormone (PTH), zinc, and BMD in zone 7. To sum up, it is found that trace elements may be related to prosthesis loosening, which provides experimental basis for the treatment of prosthesis loosening later.
