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Related Experiment Video

Updated: Dec 23, 2025

Fluorimetric Techniques for the Assessment of Sperm Membranes
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Fluorimetric Techniques for the Assessment of Sperm Membranes

Published on: November 28, 2018

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Zeta potential technique for analyzing semen quality.

Maksim Ionov1, Wojciech Gontarek2, Maria Bryszewska1

  • 1Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, Pomorska Street 141/143, Lodz 90-236, Poland.

Methodsx
|April 30, 2020
PubMed
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This pilot study explores using zeta potential to assess sperm quality. Results show a more negative sperm surface charge correlates with healthier semen, offering a simple new method for semen analysis.

Area of Science:

  • Reproductive biology
  • Biophysical techniques

Background:

  • Semen quality assessment is crucial for reproductive health.
  • Traditional semen analysis includes motility, viability, and morphology.
  • A need exists for simple, objective methods to evaluate semen parameters.

Purpose of the Study:

  • To investigate the utility of zeta potential measurement for human semen quality assessment.
  • To determine if a commercial Zeta Potential analyzer can be used for this purpose.
  • To explore the relationship between sperm surface charge and semen parameters.

Main Methods:

  • Semen samples were collected from healthy young adult male donors.
  • Samples were analyzed for motility, viability, morphology, and concentration.
  • Sperm surface charge was measured using a commercially available Zeta Potential analyzer.
Keywords:
Membrane chargeSemen analysisZeta potential method

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  • Only 20 µl of semen was required for zeta potential analysis.

    • Main Results:

    • Semen samples with optimal structural and functional parameters exhibited a significantly more negative zeta potential.
    • Sperm surface charge, measured by zeta potential, reflects key semen characteristics.
    • The study demonstrated the feasibility of using zeta potential analysis in routine semen evaluation.

    Conclusions:

    • Zeta potential measurement is a viable and simple additional method for assessing human semen quality.
    • Sperm surface charge characteristics, quantifiable via zeta potential, provide valuable insights into semen health.
    • Commercial Zeta Potential analyzers can be effectively applied to investigate sperm surface charge and semen quality.