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[Effect of osthole on periodontal remodeling during orthodontic tooth movement in rats]
Wen-Xiu Yuan1, Xu-Xia Wang, Li-Na Zhang
1Shandong Provincial Key Laboratory of Oral Tissue Regeneration and Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration. Jinan 250012, China.
Purpose:
To investigate the effect of osthole on periodontal remodeling during orthodontic tooth movement (OTM) in rats.
Methods:
Seventy two 8-week-old male SD rats were randomly equally divided into 3 groups: two experimental groups of osthole with low (20 mg/kg) and high (40 mg/kg) concentration and the control group. Models of OTM were routinely established. Rats in the experimental groups were respectively given osthole by intragastric administration, while rats in the control group received the same volume of solvent. The rats were sacrificed on 7th, 14th, 21st and 28th day after orthodontic treatment, and the maxilla was harvested and the distance between the first and second molar was measured in each stage. Hematoxylin-eosin (H-E) staining, tartrate resistant acid phosphatase (TRACP) staining were performed. The results were analyzed with SPSS 20.0 software package for one-way analysis of variance.
Results:
The mesio-moving distance of the three groups successively increased gradually. On the 7th day, there was no difference between the low concentration group and the control group (P>0.05); at other time point, the experimental groups exhibited significant differences from the control group(P<0.05), and the high concentration group had more obviously mesio-movement than the low concentration group(P<0.05). Histological observation showed that in the tension side, osteoblast appeared, but more apparent in the experimental groups than in the control group. In the pressure side, the number of osteoclast reached the peak at the 7th day, and much more osteoclasts were seen in the experimental groups than in the control group (P<0.05), in high concentration group than in low concentration group (P<0.05). The number of osteoclast decreased subsequently, but significant difference existed between the experimental groups and the control group (P<0.05) on the 14th day. At other time points, there was no significant difference among the three groups(P>0.05).
Conclusions:
Osthole could increase the number of osteoclast in periodontium and promote bone remodeling at the early stage of treatment, its effect is dose-dependence during OTM.
