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Mechanism of Virus Attenuation by Codon Pair Deoptimization.

Nicole Groenke1, Jakob Trimpert1, Sophie Merz2

  • 1Institut für Virologie, Freie Universität Berlin, Robert-von-Ostertag-Str. 7-13, 14163 Berlin, Germany.

Cell Reports
|April 30, 2020
PubMed
Summary

Codon pair deoptimization weakens viruses by reducing mRNA stability and protein production. This strategy, altering synonymous codons, offers a new approach for virus attenuation and synthetic gene optimization.

Keywords:
attenuationcodon biascodon pair biascodon pair deoptimizationdinucleotide frequenciesinfluenza A virusmRNA stabilityrecodingsynthetic attenuated virus engineering

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