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Next-Generation Sequencing and MALDI Mass Spectrometry in the Study of Multiresistant Processed Meat

Carolina Sabença1,2,3,4, Telma de Sousa1,2,3,4, Soraia Oliveira1,2,3,4

  • 1Department of Genetics and Biotechnology, University of Trás-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, Portugal.

Biology
|May 1, 2020
PubMed

Insights

Vancomycin-resistant enterococci (VRE) found in processed meat show high resistance to common antibiotics. Combined Next-Generation Sequencing and MALDI-TOF MS identified specific resistance genes and biomarkers for VRE characterization.

Area of Science:

  • Microbiology
  • Food Safety
  • Genomics

Background:

  • Vancomycin-resistant enterococci (VRE) pose treatment challenges due to antibiotic resistance and adaptable genomes.
  • VRE infections are a growing concern in healthcare and food production environments.
  • Characterizing VRE isolates is crucial for understanding resistance mechanisms and public health risks.

Purpose of the Study:

  • To characterize vancomycin-resistant enterococci (VRE) isolates from processed meat using phenotypic and genotypic methods.
  • To identify antibiotic resistance genes and potential biomarkers in VRE.
  • To evaluate the utility of Next-Generation Sequencing (NGS) and MALDI-TOF Mass Spectrometry (MS) for VRE analysis.

Main Methods:

  • Phenotypic antibiotic susceptibility testing was performed on VRE isolates.
  • Genotypic analysis using Next-Generation Sequencing (NGS) identified resistance genes.
  • MALDI-TOF Mass Spectrometry (MS) was employed for bacterial identification and biomarker discovery.

Main Results:

  • Nineteen VRE isolates exhibited high resistance to teicoplanin, tetracycline, and erythromycin.
  • NGS confirmed most isolates as vanA-positive, with prevalent erm(B) and aac(6')-Ii resistance genes.
  • MALDI-TOF MS successfully identified the Enterococcus genus and differentiated Enterococcus faecium, detecting resistance-associated protein peaks.

Conclusions:

  • Combined NGS and MALDI-TOF MS are effective complementary techniques for VRE characterization.
  • Specific resistance genes and protein biomarkers were identified in VRE isolates from processed meat.
  • This study highlights the importance of monitoring VRE in food products for public health.
Keywords:
Enterococcus spp.MALDI-TOF MSantibiotic resistancenext-generation sequencingprocessed meat

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