N-glycosylation of α_1D-adrenergic receptor N-terminal domain is required for correct trafficking, function, and...

G protein-coupled receptor (GPCR) biogenesis, trafficking, and function are regulated by post-translational modifications, including N-glycosylation of asparagine residues. α_1D-adrenergic receptors (α_1D-ARs) - key regulators of central and autonomic nervous system function - contain two putative N-glycosylation sites within the large N-terminal domain at N65 and N82. However, determining the glycosylation state of this receptor has proven challenging. Towards understanding the role of these putative glycosylation sites, site-directed mutagenesis and lectin affinity purification identified N65 and N82 as bona fide acceptors for N-glycans. Surprisingly, we also report that simultaneously mutating N65 and N82 causes early termination of α_1D-AR between transmembrane domain 2 and 3. Label-free dynamic mass redistribution and cell surface trafficking assays revealed that single and double glycosylation deficient mutants display limited function with impaired plasma membrane expression. Confocal microscopy imaging analysis and SNAP-tag sucrose density fractionation assays revealed the dual glycosylation mutant α_1D-AR is widely distributed throughout the cytosol and nucleus. Based on these novel findings, we propose α_1D-AR transmembrane domain 2 acts as an ER localization signal during active protein biogenesis, and that α_1D-AR N-terminal glycosylation is required for complete translation of nascent, functional receptor.