Whole-genome sequence and comparative genome analysis of Lactobacillus paracasei DTA93, a promising probiotic lactic...

The complete genome sequence of Lactobacillus paracasei DTA93, isolated from healthy infant feces is reported, along with in silico genetic analyses of its main features. The 3.02 Mb sequenced genome possesses 2990 protein-coding sequences distributed on 341 SEED subsystems. In previous in vitro studies, this strain demonstrated interesting probiotic properties, anti-cancer activity, and anti-bacterial activity. The whole-genome sequencing allowed to identify DTA93 as L. paracasei and to precisely place it within the L. casei group, since it shows the highest number of orthologous genes/proteins in common with the type strain L. paracasei ATCC 25302^T. In silico analyses revealed the absence of potentially negative traits, such as presence of prophages, transmissible antibiotic resistance and virulence factors. The results provided here considerably increased the amount of information available on DTA93 in favor of its possible use in food products as health-promoting culture. The complete genome data have been deposited in GenBank under the accession number VTYT00000000.