Related Experiment Video
Updated: Dec 22, 2025

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis
Published on: March 30, 2019
LINC00473 promotes lung adenocarcinoma progression by regulating miR-1294/ROBO1 axis
1The Fifth Department of Oncology, The Fourth People's Hospital of Zibo, Zibo, Shandong Province, China.
Abstract:
Long noncoding RNA (lncRNA) LINC00473 has been reported to be involved in the regulation of several human cancers. However, the regulatory mechanism of LINC00473 is still unknown in lung adenocarcinoma. In this study, RT-qPCR was used to measure the expression of LINC00473, miR-1294 and ROBO1. The functional mechanism of the LINC00473/miR-1294/ROBO1 pathway was investigated by CCK-8, Transwell and dual luciferase reporter assays. The results showed that LINC00473 was up-regulated and miR-1294 was down-regulated in lung adenocarcinoma tissues and cells. LINC00473 can bind to miR-1294, and reciprocal inhibition between LINC00473 and miR-1294 expression was identified in lung adenocarcinoma. Functionally, LINC00473 promoted cell proliferation and motility in lung adenocarcinoma by downregulating miR-1294. In addition, miR-1294 directly targets ROBO1. ROBO1 served as an oncogene in lung adenocarcinoma. In particular, LINC00473 promoted the progression of lung adenocarcinoma by upregulating ROBO1. In conclusion, LINC00473 acts as a tumor promoter in lung adenocarcinoma by regulating the miR-1294/ROBO1 axis.
Related Concept Videos
lncRNA - Long Non-coding RNAs
MicroRNAs
MicroRNAs
mTOR Signaling and Cancer Progression
The mTOR pathway or the...
Abnormal Proliferation
The Retinoblastoma Gene
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
