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Published on: March 30, 2019
miR-30a-5p Inhibits Proliferation and Migration of Lung Squamous Cell Carcinoma Cells by Targeting FOXD1
Chunhua Chen1, Junhua Tang2, Shan Xu1
1Department of Pulmonary and Critical Care Medicine, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou 310016, China.
Objective:
To investigate the mechanism of miR-30a-5p inhibiting proliferation and migration of lung squamous cell carcinoma (LSCC) cells by targeting FOXD1.
Methods:
Bioinformatics was used to analyze differentially expressed genes in the TCGA_LUSC database. qRT-PCR was used to detect the expression levels of miR-30a-5p and FOXD1 in human normal lung epithelial cell line and human LSCC cell lines. The protein expression of FOXD1 was detected by western blot. The cell viability and colony formation abilities were examined by CCK-8 and colony formation assays, respectively. Wound healing and Transwell assays were performed to examine the migration and invasion abilities of cells. The targeted binding sites of miR-30a-5p and FOXD1 were predicted by bioinformatics, and dual luciferase assay was used to verify the targeted binding relationship between miR-30a-5p and FOXD1.
Result:
miR-30a-5p was downregulated in LSCC tissues and cells, while FOXD1 was highly expressed. Overexpression of miR-30a-5p or silencing FOXD1 inhibited cell viability, colony formation ability, migration, and invasion of LSCC cells. miR-30a-5p inhibited the proliferation and migration of LSCC cells by downregulating the expression of FOXD1.
Conclusion:
miR-30a-5p can downregulate the expression of FOXD1 and inhibit the proliferation and migration of LSCC.
Insights
MicroRNA-30a-5p (miR-30a-5p) inhibits lung squamous cell carcinoma (LSCC) growth and spread by reducing FOXD1 expression. This finding offers a potential therapeutic target for LSCC treatment.
Area of Science:
- Molecular Biology
- Oncology
- Gene Regulation
Background:
- Lung squamous cell carcinoma (LSCC) is a major subtype of lung cancer with limited treatment options.
- Understanding the molecular mechanisms underlying LSCC progression is crucial for developing targeted therapies.
Purpose of the Study:
- To elucidate the role of miR-30a-5p in LSCC.
- To investigate the inhibitory mechanism of miR-30a-5p on LSCC proliferation and migration.
- To identify FOXD1 as a direct target of miR-30a-5p in LSCC.
Main Methods:
- Bioinformatic analysis of TCGA_LUSC database for differentially expressed genes.
- qRT-PCR and Western blot to assess miR-30a-5p and FOXD1 expression.
- Cell viability (CCK-8), colony formation, wound healing, and Transwell assays to evaluate cellular functions.
- Dual luciferase assay to confirm the targeting relationship between miR-30a-5p and FOXD1.
Main Results:
- miR-30a-5p was significantly downregulated in LSCC tissues and cells, whereas FOXD1 was highly expressed.
- Overexpression of miR-30a-5p or silencing FOXD1 suppressed LSCC cell viability, colony formation, migration, and invasion.
- miR-30a-5p directly targets FOXD1, leading to its downregulation and subsequent inhibition of LSCC proliferation and migration.
Conclusions:
- miR-30a-5p acts as a tumor suppressor in LSCC by downregulating FOXD1 expression.
- Targeting the miR-30a-5p/FOXD1 axis presents a potential therapeutic strategy for LSCC.
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