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Enkephalin activity on antigen-induced proliferation of human peripheral blood mononucleate cells
G Roscetti1, C M Ausiello, C Palma
1Dipartimento di Medicina Sperimentale e Scienze Biochimiche, II Universita degli Studi di Roma, Tor Vergata, Rome, Italy.
Abstract:
The effect of the two opioid pentapeptides met- and leu-enkephalin and of the tetrapeptide TYR-GLY-GLY-PHE on the stimulated proliferation of human peripheral blood mononucleate cells (PBMC) is described. Cell populations obtained from different donors were induced to proliferate with a Candida antigen. In the presence of the antigen, met-enkephalin and TYR-GLY-GLY-PHE had a concentration-dependent diphasic effect. The proliferation of PBMC was stimulated at high peptide concentration, while it was inhibited at the lower concentrations used. The effect of leu-enkephalin appears to be rather ambiguous, and directed towards the inhibition rather than the stimulation of PBMC proliferation.