Related Experiment Video
Updated: Dec 22, 2025
05:41
Therapeutic Evaluation of Fecal Microbiota Transplantation in an Interleukin 10-Deficient Mouse Model
Published on: April 6, 2022
3.3K
Disordered Gut Microbiota in Children Who Have Chronic Pancreatitis and Different Functional Gene Mutations
Wei Wang1, Yuan Xiao2, Xinqiong Wang2
1Department of General Surgery and Research Institute of Pancreatic Diseases, Ruijin Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
Clinical and Translational Gastroenterology
|May 1, 2020
Summary
Children with chronic pancreatitis (CCP) show significantly reduced gut microbial diversity. Key probiotic genera are depleted, with specific changes linked to genetic mutations, impacting disease mechanisms.
Area of Science:
- Microbiome research
- Gastroenterology
- Pediatric medicine
Background:
- Chronic pancreatitis (CP) pathogenesis is poorly understood, particularly in children (CCP).
- Gut microbiota and host genetics are implicated, but their interplay in CCP remains unclear.
- External factors are less significant in pediatric cases, highlighting intrinsic mechanisms.
Purpose of the Study:
- To identify key gut microbiota genera in children with chronic pancreatitis (CCP).
- To characterize functional gene mutations associated with CCP.
- To explore the relationship between gut microbiota composition and genetic factors in CCP.
Main Methods:
- 16S rRNA sequencing was employed to analyze gut microbiota composition.
- Comparison between healthy controls and CCP patients, including those with various functional gene mutations.
- Receiver operating characteristic (ROC) curve analysis was used to identify diagnostic microbial signatures.
Main Results:
- CCP exhibited significantly reduced alpha diversity in gut microbiota.
- Abundances of Faecalibacterium, Subdoligranulum, Phascolarctobacterium, Bifidobacterium, Eubacterium, and Collinsella were significantly decreased in CCP (AUC=0.92).
- Functional analysis revealed reduced ribosomal activity and metabolism pathways, with enrichment of phosphotransferase systems; specific genera abundances varied with CFTR, CASR, CTSB, SPINK1, and/or PRSS1 mutations.
Conclusions:
- Children with chronic pancreatitis (CCP) experience a depletion of beneficial gut microbiota.
- Distinct gut microbiota profiles are associated with specific functional gene mutations in CCP patients.
- Understanding these microbial and genetic interactions is crucial for elucidating CCP pathogenesis.
Related Concept Videos
01:24Chronic Pancreatitis I: Introduction
608
The pancreas, an elongated and flat gland situated behind the stomach, serves a vital function in digesting food and managing blood sugar levels.
Pancreatitis is the inflammation of the pancreas, which occurs when the immune system becomes active and causes swelling, pain, and disruptions in organ function. Pancreatitis can manifest as either an acute or chronic condition.
Acute pancreatitis arises suddenly and lasts for a brief duration, while chronic pancreatitis is a long-term affliction...
Pancreatitis is the inflammation of the pancreas, which occurs when the immune system becomes active and causes swelling, pain, and disruptions in organ function. Pancreatitis can manifest as either an acute or chronic condition.
Acute pancreatitis arises suddenly and lasts for a brief duration, while chronic pancreatitis is a long-term affliction...
608
01:23Cystic Fibrosis: Pathogenesis
644
Cystic fibrosis (CF), an autosomal recessive disorder, significantly affects the function of exocrine glands. This genetically inherited disease is characterized by the production of thick and sticky mucus, which can severely affect various organs and systems in the body.
CF is primarily caused by a genetic mutation in a chromosome 7 gene coding for the cystic fibrosis transmembrane conductance regulator (CFTR) protein. The most common gene mutation leading to CF is the ΔF508 mutation,...
CF is primarily caused by a genetic mutation in a chromosome 7 gene coding for the cystic fibrosis transmembrane conductance regulator (CFTR) protein. The most common gene mutation leading to CF is the ΔF508 mutation,...
644
01:17Chronic Bowel Disorders: Introduction
693
Chronic bowel diseases are a group of long-term conditions affecting the digestive tract, characterized by inflammation and damage to the gut lining. These conditions primarily include irritable bowel syndrome and inflammatory bowel disease.
Irritable Bowel Syndrome (IBS) is a common disorder affecting the gastrointestinal tract. The distinctive feature is recurrent abdominal pain associated with altered bowel movements, manifesting as constipation, diarrhea, or fluctuating between both. The...
Irritable Bowel Syndrome (IBS) is a common disorder affecting the gastrointestinal tract. The distinctive feature is recurrent abdominal pain associated with altered bowel movements, manifesting as constipation, diarrhea, or fluctuating between both. The...
693
01:29Chronic Pancreatitis II: Collaborative Care
224
The management of chronic pancreatitis is multifaceted, involving a comprehensive approach that includes thorough assessment, diagnostic testing, and a variety of management strategies.
Assessment:
Assessment:
224
01:23Anatomy of the Intestines
86.3K
Although digestion of proteins, carbohydrates, and lipids may begin in the stomach, it is completed in the intestine. The absorption of nutrients, water, and electrolytes from food and drink also occurs in the intestine. The intestines can be divided into two structurally distinct organs—the small and large intestines.
Small Intestines
The small intestine is an ~7 meter-long tube with an inner diameter of just 2.5 cm. Since most nutrients are absorbed here, the inner lining of the...
Small Intestines
The small intestine is an ~7 meter-long tube with an inner diameter of just 2.5 cm. Since most nutrients are absorbed here, the inner lining of the...
86.3K