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Related Experiment Video

Updated: Dec 22, 2025

Bioprinting Cellularized Constructs Using a Tissue-specific Hydrogel Bioink
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Injectable Therapeutic Organoids Using Sacrificial Hydrogels.

Ninna S Rossen1, Priya N Anandakumaran2, Rafael Zur Nieden2

  • 1Department of Biomedical Engineering, Columbia University, 351 Engineering Terrace, 1210 Amsterdam Avenue, New York, NY 10027, USA; Biotech Research & Innovation Centre, University of Copenhagen, University of Copenhagen, Ole MaaløesVej 5, 2200 Copenhagen N, Denmark; Department of Radiation Oncology, Stanford University, 269 Campus Drive, Palo Alto, CA 94305, USA.

Iscience
|May 1, 2020
PubMed
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This summary is machine-generated.

This study presents a scalable hydrogel scaffold method for generating reproducible organoids for cell therapy. These self-organized multicellular structures rapidly restore vascular perfusion in preclinical models.

Area of Science:

  • Biotechnology
  • Regenerative Medicine
  • Tissue Engineering

Background:

  • Organoid technology is expanding for drug screening but faces challenges in cell therapy due to limited scalability and reproducibility.
  • Current methods for producing self-organized cell clusters lack consistent size and cellular organization, hindering therapeutic applications.

Purpose of the Study:

  • To develop a scalable and reproducible method for generating self-organized multicellular structures for cell therapy.
  • To demonstrate the therapeutic potential of these engineered structures in restoring vascular function.

Main Methods:

  • Utilized sacrificial hydrogels as scaffolds to guide cell self-organization into reproducible multicellular structures.
  • Gently released the formed cell clusters for subsequent therapeutic use.
Keywords:
BioelectronicsBiomaterialsBiotechnology

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  • Tested the efficacy of endothelial and mesenchymal stem cell-derived blood-vessel units in mouse models.

    • Main Results:

    • Achieved large-scale production of highly reproducible multicellular structures.
    • Demonstrated rapid formation of perfusing vasculature upon injection of engineered blood-vessel units in mice.
    • Showcased significant restoration of vascular perfusion within seven days in a mouse model of peripheral artery disease.

    Conclusions:

    • The sacrificial hydrogel scaffold method enables high-yield, reproducible generation of organoids with controlled architecture.
    • This approach offers a promising strategy for advancing cell therapy by providing consistent, scalable multicellular aggregates.
    • The method holds potential for various therapeutic applications requiring organized cell structures.