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BRM270 Suppresses Cervical Cancer Stem Cell Characteristics and Progression by Inhibiting SOX2

Nisansala Chandimali1,2, Hu-Nan Sun3, Yang Ho Park4

  • 1Immunotherapy Convergence Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, Republic of Korea.

In Vivo (Athens, Greece)
|May 2, 2020
PubMed
Abstract

Insights

BRM270, a plant extract, effectively inhibits cervical cancer progression by targeting SOX2 and CD133 expression in cancer stem cells. This formulation demonstrates therapeutic potential by reducing proliferation and tumor growth in vitro and in vivo.

Area of Science:

  • Oncology
  • Plant-derived therapeutics
  • Cancer stem cell biology

Background:

  • Cervical cancer remains a significant global health challenge for women.
  • BRM270, a plant extract formulation, exhibits potential anticancer properties by inhibiting cell proliferation and CD133 expression in cancer stem cells.
  • Sex-determining region Y-box 2 (SOX2) is implicated in cervical cancer initiation and metastasis.

Purpose of the Study:

  • To evaluate the therapeutic efficacy of BRM270 against cervical cancer progression.
  • To investigate the effects of BRM270 on SOX2 and CD133 expression in cervical cancer cells.
  • To assess the in vitro and in vivo impact of BRM270 on cervical cancer stem cells (CSCs).

Main Methods:

  • Tested SOX2 expression in HeLa, SiHa, Caski, and C33A cervical cancer cell lines.
  • Selected SOX2-expressing SiHa and C33A cells for in vitro and in vivo experiments.
  • Utilized western blotting, flow cytometry, sphere formation assays, magnetic-activated cell sorting for CD133+ cells, and xenografts in nude mice.

Main Results:

  • BRM270 inhibited SOX2 expression in cervical cancer stem cells (CSCs).
  • BRM270 suppressed CD133 expression, induced CSC apoptosis, and inhibited proliferation and sphere formation in vitro.
  • BRM270 suppressed xenograft tumor growth in vivo and downregulated epithelial-to-mesenchymal transition markers.

Conclusions:

  • BRM270 demonstrates significant therapeutic potential for cervical cancer treatment.
  • BRM270 effectively targets key markers (SOX2, CD133) involved in cervical cancer progression.
  • Further research into BRM270 as a cervical cancer therapeutic agent is warranted.
Keywords:
BRM270SOX2cancer stem cellscervical cancerepithelial-to-mesenchymal transition

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