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Complete Genome Sequence of Brucella abortus Phage EF4, Determined Using Long-Read Sequencing
Calvin Cicha1, Jodi Hedges1, Ian Novak1
1Department of Microbiology and Immunology, Montana State University, Bozeman, Montana, USA.
Microbiology Resource Announcements
|May 2, 2020
Summary
Brucellaphage EF4, a novel virus from elk feces, has a 38,321-bp DNA genome. This phage is genetically similar to other viruses in the Perisivirus genus.
Area of Science:
- Virology
- Microbiology
- Genomics
Background:
- Brucellaphages are viruses that infect Brucella bacteria.
- Phage discovery is crucial for understanding microbial ecosystems and developing phage-based therapies.
- Elk are potential reservoirs for various microorganisms, including novel phages.
Purpose of the Study:
- To isolate and characterize a novel brucellaphage from elk feces.
- To determine the genomic features of the isolated phage.
- To establish the phylogenetic relationship of the new phage with known brucellaphages.
Main Methods:
- Isolation of bacteriophage from elk fecal samples.
- Whole-genome sequencing of the double-stranded DNA phage genome.
- Bioinformatic analysis to predict coding regions and identify functional genes.
- Comparative genomic analysis to determine nucleotide similarity with related phages.
Main Results:
- Brucellaphage EF4 was successfully isolated from elk feces.
- The double-stranded DNA genome of Brucellaphage EF4 is 38,321 base pairs in length.
- Bioinformatic analysis predicted 72 coding regions, with 38 assigned putative functions.
- Significant nucleotide similarity was observed between Brucellaphage EF4 and other brucellaphages within the genus Perisivirus.
Conclusions:
- Brucellaphage EF4 represents a newly identified member of the Perisivirus genus.
- The genomic characterization provides insights into the genetic makeup of phages found in wildlife reservoirs.
- Further studies on Brucellaphage EF4 could elucidate its biological properties and potential applications.
