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Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells

Published on: February 9, 2012

Quantitative Viscosity Mapping Using Fluorescence Lifetime Measurements.

J Dench¹, N Morgan^1,2, J S S Wong¹

¹1Department of Mechanical Engineering, Imperial College London, London, SW7 2AZ UK.

Tribology Letters

|May 2, 2020

Summary

This summary is machine-generated.

Molecular rotors measure lubricant viscosity in elastohydrodynamic (EHD) lubrication. This study validates their use, comparing results with conventional rheometry for tribology and energy efficiency applications.

Keywords:

Elastohydrodynamic lubrication Fluorescence lifetime High-pressure rheology In situ Molecular rotor Viscosity

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Area of Science:

Tribology and Lubrication Science
Materials Science and Engineering
Physical Chemistry

Background:

Lubricant viscosity critically impacts tribological performance and energy efficiency.
Elastohydrodynamic (EHD) lubrication involves extreme pressures and shear rates, challenging conventional rheometry.
In situ rheological measurements are crucial for understanding fluid behavior under EHD conditions.

Purpose of the Study:

To investigate the in situ measurement of local viscosity in EHD lubrication using molecular rotors.
To validate the application of molecular rotors for rheological measurements under EHD conditions.
To propose a calibration procedure for molecular rotors at elevated pressures.

Main Methods:

Utilized molecular rotors for in situ local viscosity measurements within a model EHD lubricant.
Employed fluorescence lifetime measurements correlated with viscosity.
Compared molecular rotor data with reference measurements from conventional rheometry techniques.
Verified standard tribological methods (friction and film thickness) for homogeneous EHD flow.

Main Results:

Demonstrated the validity of molecular rotors for measuring local viscosity in EHD lubrication.
Showcased good agreement between molecular rotor measurements and conventional rheometry.
Confirmed the appropriateness of standard tribological methods under specific flow conditions.
Proposed a straightforward calibration procedure for molecular rotors at high pressures.

Conclusions:

Molecular rotors offer a viable method for in situ viscosity determination in EHD lubrication.
The findings support the use of molecular rotors to study lubricant behavior under extreme conditions.
Standard tribological techniques are reliable for homogeneous EHD flow, but molecular rotors provide complementary insights.
The proposed calibration method simplifies the application of molecular rotors for high-pressure viscosity measurements.