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miR-566 expression and immune changes in patients with intracranial aneurysm
Hongjun Fan1, Chun Yang1, Chenguang Jia1
1Department of Neurosurgery, Zhuzhou Central Hospital Zhuzhou, Hunan, China.
Objective:
Our study aims to investigate the correlations of micro ribonucleic acid (miR)-566 expression with the changes in immune-related indexes and differential genes in patients with intracranial aneurysm (IA).
Methods:
Aneurysm wall tissues from a total of 50 IA cases and the corresponding normal arterial wall tissues from 50 individuals were selected. The miR-566 expression, differential gene expression profile, and expression level of differential gene proteins were detected and analyzed by fluorescence quantitative polymerase chain reaction (qPCR), RNAseq technique and western blotting, respectively.
Results:
The miR-566 level was significantly higher in intracranial aneurysm tissues than that in normal arterial wall tissues (P<0.05). The levels of cluster of differentiation (CD)3+, CD4+, CD8+, CD4+/CD8+ and CD23+ T lymphocytes in the peripheral blood of IA patients significantly declined compared with those in the control group (P<0.05). RNAseq detection showed that there were 16 immune-inflammation-related genes significantly differentially expressed in aneurysm wall tissues compared with normal arterial wall tissues in the control group. The levels of VHL and NIK in aneurysm wall tissues were significantly decreased, while those of VEGF and ALOX5 were obviously increased. Both mRNA and protein levels of these four genes also had significant changes, which had linear relations to the expression of miR-566.
Conclusion:
The abnormal expression of miR-566 affects the immune function, thus promoting the occurrence and deterioration of intracranial aneurysm.
