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Related Experiment Videos

Determination of polycyclic aromatic compounds in fish tissue.

D A Birkholz1, R T Coutts, S E Hrudey

  • 1Enviro-Test Laboratories, Edmonton, Alberta, Canada.

Journal of Chromatography
|September 30, 1988
PubMed
Summary

This study presents a new method for analyzing polycyclic aromatic hydrocarbons (PAHs), PASHs, and PANHs in fish. The validated technique ensures accurate detection of these contaminants in fish tissue samples.

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Area of Science:

  • Environmental Chemistry
  • Analytical Chemistry
  • Food Safety

Background:

  • Polycyclic Aromatic Hydrocarbons (PAHs), polycyclic aromatic sulfur heterocycles (PASHs), and basic polycyclic aromatic nitrogen heterocycles (PANHs) are environmental contaminants.
  • Accurate analysis of these compounds in fish is crucial for assessing food safety and environmental impact.

Purpose of the Study:

  • To develop and validate a comprehensive analytical method for the simultaneous determination of PAHs, PASHs, and basic PANHs in fish tissue.
  • To ensure the method effectively removes interfering biogenic compounds for reliable quantification.

Main Methods:

  • Soxhlet extraction of fish tissue using methylene chloride.
  • Gel permeation chromatography (GPC) for initial cleanup.
  • Adsorption chromatography (Florisil) for PAHs/PASHs and liquid-liquid partitioning for basic PANHs.

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  • Analysis by capillary gas chromatography with flame ionization detection (GC-FID) and gas chromatography-mass spectrometry (GC-MS).

    • Main Results:

    • The method demonstrated good agreement between GC-FID and GC-MS analyses for fortified samples.
    • Average recoveries for PAHs/PASHs ranged from 70% to 87%, depending on fortification levels.
    • Average recovery for basic PANHs was high at 97%.

    Conclusions:

    • The developed analytical procedure is effective for analyzing PAHs, PASHs, and basic PANHs in fish.
    • The method's ability to remove interfering compounds ensures reliable results for environmental monitoring and food safety assessments.