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Updated: Dec 22, 2025
High-throughput Detection Method for Influenza Virus
Published on: February 4, 2012
A strip test for the optical determination of influenza virus H3 subtype using gold nanoparticle coated polystyrene
Xiao Liu1,2,3, Jifei Yang2, Qingmei Li2
1College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450002, China.
Abstract:
A strip test is described for the optical determination of influenza virus H3 subtype. It utilizes gold nanoparticle (AuNP) coated polystyrene latex microspheres (PS) as the label and a sandwich format. The AuNP and PS particles were linked using monoclonal antibodies against influenza virus as the bridge. Under the optimal conditions, the visual detection limit of the AuNP-PS-based strip test was as low as 1/16 hemagglutination unit (HAU). It was 64 times higher than that of 10 nm (4 HAU) AuNP-based strip tests. Quantitative analysis showed that the detection limit of the AuNP-PS-based strip is 0.016 HAU. The AuNP-PS-based strip test showed no cross-reactivity to the other subtypes (H1, H5, H7, or H9) of influenza viruses. Graphical abstract .
