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Biofilm Eradication Using Biogenic Silver Nanoparticles.

María Belén Estevez1, Sofía Raffaelli1, Scott G Mitchell2,3

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This study produced silver nanoparticles using Phanerochaete chrysosporium extracts, demonstrating their antimicrobial and antibiofilm capabilities against E. coli and C. albicans by disrupting cell membranes.

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antibiofilmantimicrobialbiogenic nanoparticlessilver nanoparticles

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Area of Science:

  • Biotechnology
  • Materials Science
  • Microbiology

Background:

  • Microorganisms offer green synthesis routes for valuable products.
  • Fungi produce bioactive compounds that act as reducing and stabilizing agents for metal nanoparticles.
  • Silver nanoparticles exhibit antimicrobial properties with diverse industrial applications.

Purpose of the Study:

  • To synthesize silver nanoparticles using cell-free extracts of Phanerochaete chrysosporium.
  • To evaluate the antimicrobial and antibiofilm activity of synthesized silver nanoparticles.
  • To investigate the interaction mechanisms between silver nanoparticles and E. coli.

Main Methods:

  • Synthesis of silver nanoparticles using extracellular cell-free extracts of Phanerochaete chrysosporium.
  • Characterization of silver nanoparticles' size, surface charge, and colloidal stability.
  • Analysis of nanoparticle-E. coli interactions using environmental SEM, TEM, and confocal Raman microscopy.

Main Results:

  • Synthesized 45-nm silver nanoparticles with a -30 mV surface charge, stable from pH 3-9 and up to 500 mM NaCl.
  • Observed disruption of E. coli cell membrane integrity, evidenced by decreased fatty acid content.
  • Demonstrated biofilm disruption activity against E. coli and C. albicans.

Conclusions:

  • Phanerochaete chrysosporium is effective for green synthesis of stable silver nanoparticles.
  • These nanoparticles possess significant antimicrobial and antibiofilm properties.
  • The mechanism involves disruption of bacterial cell membrane integrity.