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Updated: Dec 22, 2025

Bacterial Expression and Purification of Human Matrix Metalloproteinase-3 using Affinity Chromatography
Published on: March 30, 2022
Structure and Function of Human Matrix Metalloproteinases
Helena Laronha1,2, Jorge Caldeira1,2
1Centro de investigação interdisciplinar Egas Moniz, Instituto Universitário Egas Moniz, 2829 Caparica, Portugal.
Abstract:
The extracellular matrix (ECM) is a macromolecules network, in which the most abundant molecule is collagen. This protein in triple helical conformation is highly resistant to proteinases degradation, the only enzymes capable of degrading the collagen are matrix metalloproteinases (MMPs). This resistance and maintenance of collagen, and consequently of ECM, is involved in several biological processes and it must be strictly regulated by endogenous inhibitors (TIMPs). The deregulation of MMPs activity leads to development of numerous diseases. This review shows MMPs complexity.
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