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Detection of vasoactive intestinal polypeptide messenger RNA in ganglioneuroblastoma by in situ hybridization

Masaru Yoshioka1, Isao Nagano1, Shozo Nakamura1

  • 1Department of Neurology, Institute of Brain Diseases (MK,IN,SN) Department of Pediatrics (MI) and Department of Pathology (NK), Tohoku University School of Medicine, Sendai, Japan.

Endocrine Pathology
|May 3, 2020
PubMed

Keywords:
Endocrine Pathology VolumeGanglionic CellVasoactive Intestinal PeptideVasoactive Intestinal PolypeptideWatery Diarrhea

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In-situ Hybridization02:31

In-situ Hybridization

In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
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