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Synthesis and rapid purification of UDP-[6-3H]galactose
B Fudem-Goldin1, P Voulalas, G A Orr
1Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, NY 10461.
Journal of Biochemical and Biophysical Methods
|November 1, 1988
Abstract:
UDP[6-3H]galactose of high specificity can be obtained by oxidation of the C-6 hydroxymethyl group of UDP-galactose by galactose oxidase and subsequent reduction by sodium borotritide. One-step purification of the nucleotide sugar involves anion-exchange chromatography on a Pharmacia Mono Q column. Radiolabeled UDP-N-acetylgalactosamine can also be synthesized and purified by this procedure. Both nucleotide sugars can be used for sugar incorporation studies using the appropriate glycosyltransferase.