Detecting β-Galactosidase-Labeled Cells
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Updated: Dec 22, 2025

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Tracing Gene Expression Through Detection of β-galactosidase Activity in Whole Mouse Embryos
Published on: June 26, 2018
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Cold Spring Harbor Protocols
|May 3, 2020
Abstract:
β-Galactosidase has been used extensively both as a label in enzyme immunoassays and for immunocytochemistry. One good substrate is 5-bromo-4-chloro-3-indolyl-β-d-galactopyranoside (X-gal), which gives an intense blue product. The product is stable and insoluble in alcohol as well as H2O.

