Detecting β-Galactosidase-Labeled Cells

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Tracing Gene Expression Through Detection of β-galactosidase Activity in Whole Mouse Embryos
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Tracing Gene Expression Through Detection of β-galactosidase Activity in Whole Mouse Embryos

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Scott J Rodig

    Cold Spring Harbor Protocols
    |May 3, 2020
    PubMed

    Abstract:

    β-Galactosidase has been used extensively both as a label in enzyme immunoassays and for immunocytochemistry. One good substrate is 5-bromo-4-chloro-3-indolyl-β-d-galactopyranoside (X-gal), which gives an intense blue product. The product is stable and insoluble in alcohol as well as H2O.

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