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Related Experiment Video

Updated: Dec 22, 2025

Corneal Donor Tissue Preparation for Descemet's Membrane Endothelial Keratoplasty
10:46

Corneal Donor Tissue Preparation for Descemet's Membrane Endothelial Keratoplasty

Published on: September 17, 2014

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Optimizing pre-Descemet endothelial keratoplasty technique.

Andrew R Ross1, Dalia G Said, Rossella A M Colabelli Gisoldi

  • 1From the Department of Ophthalmology, Nottingham University Hospitals NHS Trust and Section of Academic Ophthalmology, Division of Clinical Neuroscience, University of Nottingham (Ross, Said, Dua), Nottingham, United Kingdom; Department of Ophthalmology, Aswan University (Ross, El-Amin, Gabr, Moniem), Aswan, Egypt; Hospital San Giovanni Addolorata (Colabelli Gisoldi, Pocobelli), Rome, Department of Medicine and Science of Ageing, Ophthalmic Clinic, National High Technology Eye Center, G. d'Annunzio University of Chieti (Nubile, Mastropasqua), Pescara, Department of Oto-Neuro-Ophthalmological Surgical Sciences, Eye Clinic, University of Florence (Mencucci), Florence, Italy.

Journal of Cataract and Refractive Surgery
|May 3, 2020
PubMed
Summary
This summary is machine-generated.

Pre-Descemet endothelial keratoplasty (PDEK) offers a simpler alternative to DMEK. PDEK preparation is reproducible, yielding a consistent big bubble, and the tissue is easier to handle during surgery.

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Area of Science:

  • Ophthalmology
  • Corneal Surgery
  • Tissue Engineering

Background:

  • Descemet membrane endothelial keratoplasty (DMEK) is a common endothelial keratoplasty technique.
  • Challenges in DMEK preparation and tissue handling exist.
  • Pre-Descemet endothelial keratoplasty (PDEK) has emerged as a viable alternative.

Purpose of the Study:

  • To describe the technique and outcomes of Pre-Descemet endothelial keratoplasty (PDEK).
  • To evaluate the feasibility and advantages of PDEK compared to DMEK.
  • To highlight PDEK's potential benefits for surgeons transitioning to endothelial keratoplasty.

Main Methods:

  • Pneumatic dissection for PDEK tissue preparation using a specialized clamp.
  • Achieving a type 1 big bubble with a mean size of 7.255 × 6.745 mm using 0.14–0.37 mL air.
  • Tissue dissection via trephination or manual excision and insertion by injection or pulling.

Main Results:

  • PDEK tissue preparation is simple and reproducible.
  • Consistent type 1 big bubble formation is achievable.
  • PDEK tissue demonstrates easier handling and unscrolling compared to DMEK, allowing younger donor use.

Conclusions:

  • PDEK is a practical alternative to DMEK with significant advantages.
  • The technique offers improved preparation, handling, and unscrolling properties.
  • PDEK may facilitate surgeon adoption of endothelial keratoplasty procedures.