Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

1.4K
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
1.4K
Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

2.1K
Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
2.1K
Mass Spectrometers01:16

Mass Spectrometers

8.0K
This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
8.0K
High-Resolution Mass Spectrometry (HRMS)01:15

High-Resolution Mass Spectrometry (HRMS)

2.2K
The resolution of a mass spectrometer depends on the efficiency of separating ions with different ion masses. The mass of an atom is approximated to the sum of the masses of protons and neutrons inside, considering the masses of protons and neutrons as equal. However, the masses of the proton (1.6726 × 10−24 g) and neutron (1.6749 × 10−24 g) are not truly equal. There is a minor error in the expression of atomic masses relative to the simplest atom of hydrogen. For...
2.2K
Electrospray Ionization (ESI) Mass Spectrometry01:12

Electrospray Ionization (ESI) Mass Spectrometry

1.9K
Higher molecular weight biomolecules are nonvolatile compounds that may decompose before ionizing or vaporizing during mass analysis with conventional electron impact ionization methods. Accordingly, electrospray ionization (ESI) is the favored method for vaporizing and ionizing biomolecules as it circumvents rapid fragmentation and enables the recording of mass signals for the entire biomolecule.
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...
1.9K
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

8.0K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
8.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Phase-Specific Parameter Estimation in Chiral HPLC Using 1-Site, 2-Site Stochastic Models, and Unified Equation Approach.

Analytical chemistry·2026
Same author

Organocatalyzed bottom-up formation of protocells.

Nature communications·2026
Same author

Dual-Mode Thio-MacMillan Organocatalysts: Stereoselective Diels-Alder Reactions or Sacrificial Self-Cyclization to N-Bridged Bicyclic Lactams.

Chemistry (Weinheim an der Bergstrasse, Germany)·2025
Same author

Isotopic chirality and high-resolution gigahertz and terahertz spectroscopy of <i>trans</i>-2,3-dideutero-oxirane (<i>tc</i>-CHDCHDO).

Physical chemistry chemical physics : PCCP·2025
Same author

RaabPhos, an Air-Stable, Highly Active Ferrocenyl Phosphasilinane Ligand, for Palladium-Catalyzed C-C and C-N Bond-Forming Cross-Coupling Reactions.

Organic letters·2025
Same author

Mechanistic analysis and kinetic profiling of Soai's asymmetric autocatalysis for pyridyl and pyrimidyl substrates.

Nature communications·2025

Related Experiment Video

Updated: Dec 22, 2025

Sheathless Capillary Electrophoresis&#8211;Mass Spectrometry for Metabolic Profiling of Biological Samples
07:46

Sheathless Capillary Electrophoresis–Mass Spectrometry for Metabolic Profiling of Biological Samples

Published on: October 1, 2016

12.1K

A robust sheath-flow CE-MS interface for hyphenation with Orbitrap MS.

Fabian Sauer1, Constanze Sydow1, Oliver Trapp1,2

  • 1Department Chemie, Ludwig-Maximilians-Universität München, München, Germany.

Electrophoresis
|May 3, 2020
PubMed
Summary
This summary is machine-generated.

A new sheath-flow capillary electrophoresis-electrospray ionization-mass spectrometry (CE-ESI-MS) interface with a gold-coated emitter enhances stability and lifetime. This robust system achieves sensitive compound identification using high-resolution Orbitrap MS detection.

Keywords:
CE-MSElectrospray ionizationPeptide separation

More Related Videos

Cell-Lineage Guided Mass Spectrometry Proteomics in the Developing Frog Embryo
09:18

Cell-Lineage Guided Mass Spectrometry Proteomics in the Developing Frog Embryo

Published on: April 21, 2022

2.1K
Capillary Electrophoresis-based Hydrogen/Deuterium Exchange for Conformational Characterization of Proteins with Top-down Mass Spectrometry
05:45

Capillary Electrophoresis-based Hydrogen/Deuterium Exchange for Conformational Characterization of Proteins with Top-down Mass Spectrometry

Published on: June 8, 2021

3.6K

Related Experiment Videos

Last Updated: Dec 22, 2025

Sheathless Capillary Electrophoresis&#8211;Mass Spectrometry for Metabolic Profiling of Biological Samples
07:46

Sheathless Capillary Electrophoresis–Mass Spectrometry for Metabolic Profiling of Biological Samples

Published on: October 1, 2016

12.1K
Cell-Lineage Guided Mass Spectrometry Proteomics in the Developing Frog Embryo
09:18

Cell-Lineage Guided Mass Spectrometry Proteomics in the Developing Frog Embryo

Published on: April 21, 2022

2.1K
Capillary Electrophoresis-based Hydrogen/Deuterium Exchange for Conformational Characterization of Proteins with Top-down Mass Spectrometry
05:45

Capillary Electrophoresis-based Hydrogen/Deuterium Exchange for Conformational Characterization of Proteins with Top-down Mass Spectrometry

Published on: June 8, 2021

3.6K

Area of Science:

  • Analytical Chemistry
  • Separation Science
  • Mass Spectrometry

Background:

  • Hyphenation of capillary electrophoresis (CE) with high-resolution mass spectrometry (HRMS) is crucial for sensitive compound identification.
  • Existing interfaces face challenges with emitter tip oxidation and maintaining separation voltage stability.

Purpose of the Study:

  • To design and characterize a robust sheath-flow CE-ESI-MS interface for high-resolution Orbitrap MS.
  • To improve the stability and operational lifetime of the ionization interface.

Main Methods:

  • Development of a sheath-flow interface incorporating a gold-coated electrospray emitter.
  • Investigation of spray stability and durability under varying sheath-flow rates and electrospray voltages.
  • Optimization of gold coating for enhanced emitter performance.

Main Results:

  • The gold-coated emitter significantly increased system stability and lifetime.
  • Optimized interface conditions were determined for robust operation.
  • Demonstrated sensitive detection of angiotensin II and neurotensin with limits of detection (LODs) of 2.4 and 3.5 ng/mL, respectively.

Conclusions:

  • The developed sheath-flow CE-ESI-MS interface offers a stable and durable solution for sensitive compound analysis.
  • The gold-coated emitter design effectively addresses emitter oxidation issues.
  • This interface is well-suited for coupling with high-resolution Orbitrap MS for demanding analytical applications.