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Oxygen activation and ligand binding by pure heme-octapeptide microperoxidase-8 (MP-8)
P A Adams1, M P Byfield, R C Milton
1MRC Biomembrane Research Unit, University of Cape Town, Medical School, Republic of South Africa.
Journal of Inorganic Biochemistry
|November 1, 1988
Abstract:
The rapid, efficient preparation of pure microperoxidase-8 (MP-8) is described. Ligand binding studies confirm that MP-8 is monomeric in alkaline solution. It is shown that the monomeric MP-8 activates oxygen in a similar manner to that already reported for alkaline hemin, establishing the octapeptide as a possible second generation model for the oxygen activation/insertion reactions of the cytochrome P-450.