Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

COP Coated Vesicles00:59

COP Coated Vesicles

16.7K
Membrane-enclosed structures called vesicles transport proteins and lipids across the cell. The vesicles derive their cargo from the plasma membrane, Golgi, ER, or endosome. Coated vesicles are spherical, protein-coated carriers with a 50–100 nm diameter that mediate bidirectional transport between the ER and the Golgi. The distribution of proteins between the ER and Golgi complex is dynamic and is maintained by different coated vesicles. Their formation is driven by the assembly of...
16.7K
The Nucleosome Core Particle01:12

The Nucleosome Core Particle

2.0K
Nucleosomes are the DNA-histone complex, where the DNA strand is wound around the histone core. The histone core is an octamer containing two copies of H2A, H2B, H3, and H4 histone proteins.
Nucleosomes, paradoxically, perform two opposite functions simultaneously. On the one hand, their primary aim is to protect the delicate DNA strands from physical damage and help achieve a higher compaction ratio. On the other hand, they must allow polymerase enzymes to access histone-bound DNA during...
2.0K
The Nucleosome Core Particle02:10

The Nucleosome Core Particle

13.9K
Nucleosomes are the DNA-histone complex, where the DNA strand is wound around the histone core. The histone core is an octamer containing two copies of H2A, H2B, H3, and H4 histone proteins.
The paradox
Nucleosomes, paradoxically, perform two opposite functions simultaneously. On the one hand, their main responsibility is to protect the delicate DNA strands from physical damage and help achieve a higher compaction ratio. While on the other hand, they must allow polymerase enzymes to access DNA...
13.9K
Colloidal precipitates01:09

Colloidal precipitates

4.4K
The high insolubility of some precipitates can result in an unfavorable relative supersaturation. This can lead to colloidal particles with a large surface-to-mass ratio, where adsorption is promoted. For instance, in the precipitation of silver chloride, silver ions are adsorbed on the surface of the colloidal particles, forming a primary layer. This layer attracts ions of opposite charge (such as nitrate ions), forming a diffuse secondary layer of adsorbed ions. This electric double layer...
4.4K
Structure of Porins01:21

Structure of Porins

3.7K
Mitochondria, chloroplasts, and gram-negative bacteria have transmembrane, beta-barrel proteins called porins to mediate the free diffusion of ions and metabolites across the membrane. Mitochondrial porin precursors contain conserved amino acid sequences called beta signals at their C-terminal. Beta signals have a  motif of PoXGXXHyXHy (Po-Polar, X-Any amino acid, G-Glycine, Hy-LargeHydrophobic), which are crucial for precursor recognition to initiate precursor assembly. Beta-barrel...
3.7K
Lipids as Anchors01:32

Lipids as Anchors

7.0K
In the plasma membrane, the lipids forming the bilayer can also act as an anchor to tether proteins to the membrane. The three main types of lipid anchors found in eukaryotes are – prenyl groups, fatty acyl groups, and glycosylphosphatidylinositol or GPI groups. Prenyl and fatty acyl groups act as anchors on the cytosolic surface of the membrane, whereas GPI anchors proteins on the extracellular side.
The carboxy-terminal of most of the prenylated proteins, such as Ras proteins, contains...
7.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Comparison of rate models for gradient elution chromatography and experimental evaluation for IEC, HIC, and RPC systems.

Journal of chromatography. A·2026
Same author

Integrating hybrid modeling and high throughput screening: A modular process development platform for flowthrough chromatography.

Journal of chromatography. A·2026
Same author

Heterologous expression and functional characterization of recombinant arenin to assess its anticancer and wound-healing potential.

Bioresources and bioprocessing·2025
Same author

Influence of resin structure on the prediction of two-component protein adsorption behavior in anion exchange resins from single component batch data.

Journal of chromatography. A·2025
Same author

Higher confluency enhances lipid droplet size, triglyceride content and endocrine function on white-like adipocytes.

Archives of biochemistry and biophysics·2025
Same author

Generation of MCF-7 Spheroids in Polyethylene Glycol-Dextran Droplets for Cancer Niche Studies Using Aqueous Two-Phase System-3D Platforms.

Biotechnology journal·2025

Related Experiment Video

Updated: Dec 22, 2025

Microfluidic Fabrication of Core-Shell Microcapsules carrying Human Pluripotent Stem Cell Spheroids
10:51

Microfluidic Fabrication of Core-Shell Microcapsules carrying Human Pluripotent Stem Cell Spheroids

Published on: October 13, 2021

3.5K

Structure and functional properties of Capto™ Core 700 core-shell particles.

Calef Sánchez-Trasviña1, Preston Fuks2, Christiane Mushagasha3

  • 1Tecnologico de Monterrey, Escuela de Ingeniería y Ciencias, Centro de Biotecnología-FEMSA, Av. Eugenio Garza Sada 2501 Sur, Monterrey NL 64849, Mexico; Department of Chemical Engineering, University of Virginia, Charlottesville, VA 22904-4741, USA.

Journal of Chromatography. A
|May 4, 2020
PubMed
Summary

Capto™ Core 700 resin effectively purifies large biomolecules like bovine serum albumin (BSA) and thyroglobulin (Tg). This core-shell chromatographic support demonstrates high binding capacities and stable performance in high salt conditions, optimizing downstream processes.

Keywords:
Core-shell particlesFlow-through purificationMass transferModeling

More Related Videos

Assembly and Characterization of Polyelectrolyte Complex Micelles
08:44

Assembly and Characterization of Polyelectrolyte Complex Micelles

Published on: March 2, 2020

11.4K
Encapsulation and Permeability Characteristics of Plasma Polymerized Hollow Particles
09:27

Encapsulation and Permeability Characteristics of Plasma Polymerized Hollow Particles

Published on: August 16, 2012

11.1K

Related Experiment Videos

Last Updated: Dec 22, 2025

Microfluidic Fabrication of Core-Shell Microcapsules carrying Human Pluripotent Stem Cell Spheroids
10:51

Microfluidic Fabrication of Core-Shell Microcapsules carrying Human Pluripotent Stem Cell Spheroids

Published on: October 13, 2021

3.5K
Assembly and Characterization of Polyelectrolyte Complex Micelles
08:44

Assembly and Characterization of Polyelectrolyte Complex Micelles

Published on: March 2, 2020

11.4K
Encapsulation and Permeability Characteristics of Plasma Polymerized Hollow Particles
09:27

Encapsulation and Permeability Characteristics of Plasma Polymerized Hollow Particles

Published on: August 16, 2012

11.1K

Area of Science:

  • Bioseparation and Chromatography
  • Protein Purification
  • Downstream Processing

Background:

  • Core-shell chromatography supports offer unique properties for biomolecule purification.
  • Capto™ Core 700 is designed for flow-through purification of large biomolecules and bioparticles.
  • Characterization of resin structure and function is crucial for process optimization.

Purpose of the Study:

  • To characterize the structural and functional properties of Capto™ Core 700 resin.
  • To evaluate its performance in purifying large model impurity proteins, bovine serum albumin (BSA) and thyroglobulin (Tg).
  • To provide data for predicting and optimizing downstream purification processes.

Main Methods:

  • Structural characterization of agarose-based beads, including bead size, shell thickness, and pore radius (using inverse size exclusion chromatography).
  • Determination of binding isotherms and maximum binding capacities for BSA and Tg.
  • Assessment of resin performance under high salt conditions (500 mM NaCl).
  • Measurement of effective pore diffusivity and dynamic binding capacity (DBC10%) for both model proteins.

Main Results:

  • Capto™ Core 700 exhibits a fibrous core-shell structure with an average bead size of 90.7 μm and a shell thickness of 4.18 μm.
  • Maximum binding capacities were 55 mg/mL for BSA and 105 mg/mL for Tg.
  • Binding capacity decreased by less than 50% in 500 mM NaCl, indicating robust performance in high salt.
  • Effective pore diffusivities were lower in the core than the shell due to protein hindrance.
  • Dynamic binding capacities (DBC10%) at 2 min residence time were 24 mg/mL for BSA and 2 mg/mL for Tg.

Conclusions:

  • Capto™ Core 700 resin is well-characterized structurally and functionally for large biomolecule purification.
  • The resin demonstrates high binding capacities and tolerance to high salt concentrations.
  • Quantitative data on binding and diffusivity support its application in optimizing downstream bioprocesses for large biomolecules.