β-Carotene and its physiological metabolites: Effects on oxidative status regulation and genotoxicity in in vitro...

Carotenoids are ubiquitously distributed in nature, β-carotene being the most frequently found carotenoid in the human diet. In the human body, β-carotene is absorbed, distributed and metabolized by enzymatic and/or non-enzymatic oxidant cleavage into several metabolites. Despite the broadly accepted biological value of β-carotene, it has also been considered a double-edged sword, mainly due to its potential antioxidant versus pro-oxidant behaviour. In this sense, the aim of this work was to scrutinize the antioxidant or pro-oxidant potential of β-carotene and its metabolites, namely trans-β-apo-8'-carotenal and β-ionone. Several parameters were evaluated in this study, viz. their effects on reactive species production, both in human whole blood and neutrophils; their effects on lipid peroxidation, in the absence and presence of peroxynitrite anion (ONOO^-) or hydrogen peroxide (H₂O₂), using a synaptosomal model; and finally, their putative genotoxic effects in the human hepatic HepG2 cell line. In general, depending on the cellular model and conditions tested, β-carotene and its metabolites revealed antioxidant effects to varying degrees without significant pro-oxidant or genotoxic effects.