Polyplexes for gene and nucleic acid delivery: Progress and bottlenecks

Gene and nucleic acid delivery constitute a huge biological challenge and several attempts have been made by research laboratories to address this issue. Cationic polymers and cationic lipids (positively charged carriers) can be utilized for the transport of these biomolecules. Polyplexes (PPs) are interpolyelectrolyte complexes which are spontaneously formed through the electrostatic condensation between nucleic acid and a cationic polymer. PPs are capable of high-density payload condensation leading to cell internalization and subsequent protection from enzymatic degradation. Most cationic polymers can cross extracellular barriers, but it is more challenging to overcome intracellular barriers (efficient disassembly and endosomal escape). In this review, the use of PPs for gene and nucleic acid delivery is discussed.