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Related Experiment Video

Updated: Dec 22, 2025

Assembly and Characterization of Polyelectrolyte Complex Micelles

Assembly and Characterization of Polyelectrolyte Complex Micelles

Published on: March 2, 2020

Polyplexes for gene and nucleic acid delivery: Progress and bottlenecks.

¹College of Pharmacy Touro University, Mare Island-Vallejo California, CA, 94592, USA.

European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences

|May 4, 2020

Summary

Polyplexes (PPs), formed from cationic polymers and nucleic acids, offer a promising method for gene delivery. Overcoming intracellular barriers remains a key challenge for effective delivery and therapeutic applications.

Keywords:

Gene delivery Nucleic acid delivery Polymer Polyplex

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Area of Science:

Biotechnology
Molecular Biology
Polymer Science

Background:

Gene and nucleic acid delivery is a significant biological challenge.
Cationic polymers and lipids are explored as carriers for biomolecules.
Polyplexes (PPs) are formed via electrostatic condensation between nucleic acids and cationic polymers.

Purpose of the Study:

To review the application of polyplexes (PPs) in gene and nucleic acid delivery.
To highlight the capabilities and challenges of PPs in biomolecule transport.

Main Methods:

Review of existing literature on polyplexes for gene delivery.
Analysis of polyplex formation, payload condensation, and cellular uptake mechanisms.

Main Results:

Polyplexes enable high-density payload condensation and protection from enzymatic degradation.
Cationic polymers facilitate crossing extracellular barriers.
Efficient disassembly and endosomal escape present intracellular barriers.

Conclusions:

Polyplexes show potential for gene and nucleic acid delivery.
Further research is needed to overcome intracellular barriers for enhanced therapeutic efficacy.