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Skin tape proteomics identifies pathways associated with transepidermal water loss and allergen polysensitization in

Elena Goleva1, Agustin Calatroni2, Petra LeBeau2

  • 1Department of Pediatrics, National Jewish Health, Denver, Colo.

The Journal of Allergy and Clinical Immunology
|May 4, 2020
PubMed

Insights

Skin tape strip (STS) analysis identified a unique protein signature in children with atopic dermatitis (AD) and food allergy (FA). This signature, linked to skin barrier dysfunction, persists into adulthood, suggesting a specific endotype for AD with FA.

Area of Science:

  • Dermatology
  • Allergology
  • Proteomics

Background:

  • Atopic dermatitis (AD) and food allergy (FA) are frequently co-occurring conditions.
  • Skin barrier dysfunction is a hallmark of AD and a potential pathway for allergen sensitization.
  • Novel biomarkers are crucial for evaluating interventions targeting skin barrier defects in AD and FA.

Purpose of the Study:

  • To identify skin biomarkers in nonlesional skin of children with AD and FA.
  • To investigate the association between skin proteomic profiles and clinical phenotypes of AD and FA.
  • To validate identified biomarkers in an independent adult cohort.

Main Methods:

  • Collected skin tape strip (STS) samples from nonlesional skin of children with AD and FA, AD without FA, and nonatopic controls.
  • Performed mass spectrometry-based proteomic analysis on STS samples.
  • Assessed transepidermal water loss (TEWL) and allergic sensitization.
  • Validated proteomic findings in an independent cohort of adult AD patients with and without FA.

Main Results:

  • Identified a distinct cluster of 45 proteins (PC1) highly expressed in STS from children with AD and FA.
  • PC1 proteins correlated significantly with TEWL and allergic sensitization.
  • PC1 proteins included keratin intermediate filaments, inflammatory mediators (S100 proteins, alarmins), and metabolic enzymes.
  • Differential expression of PC1 proteins was confirmed in adult AD patients with a history of peanut allergy.

Conclusions:

  • Discovered a unique STS proteomic endotype in children with AD and FA, characterized by PC1 proteins.
  • This endotype appears to persist into adulthood, suggesting a stable molecular signature.
  • PC1 proteins are implicated in skin barrier abnormalities and may contribute to epicutaneous sensitization to food allergens.
Abstract

Keywords:
Atopic dermatitisIgEfood allergyproteomicsskin barriertransepidermal water loss

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