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Published on: July 8, 2020
22β-hydroxytingenone reduces proliferation and invasion of human melanoma cells
Elenn Suzany Pereira Aranha1, Emerson Lucena da Silva2, Felipe Pantoja Mesquita2
1Faculty of Pharmaceutical Sciences, Post Graduate Program in Biodiversity and Biotechnology of the Amazon (Bionorte), Federal University of Amazonas, Manaus, Amazonas, Brazil.
Abstract:
Melanoma is a skin cancer with high invasive potential and high lethality. Considering that quinonemethide triterpenes are described as promising anticancer agents, the aim of this study was to evaluate the effect of 22β-hydroxytingenone (22-HTG) against human melanoma cells. Alamar blue assay was performed in order to evaluate its cytotoxic effect. Thus, subtoxic concentrations (1.0, 2.0, and 2.5 μM) were used to evaluate the effect of this compound on proliferation, migration, metabolism, and invasion. IC50 value against SK-MEL-28 cell line was 4.35, 3.72, and 3.29 μM after 24, 48, and 72 h of incubation, respectively. 22-HTG reduced proliferation, migration and invasion by melanoma cells, with decreased activity of metalloproteinases (MMP-2 and MMP-9). Futhermore, 22-HTG decreased expression of lactate dehydrogenase (LDHA), an enzyme associated with cell metabolism. Howerver, the small reduction in LDHA enzyme activity must have occurred by the cytotoxic effect of 22-HTG. According to the results, 22-HTG interferes with important characteristics of cancer, with anti-proliferative, and anti-invasive effect against melanoma cells. The data suggest that 22-HTG is an effective substance against melanoma cells and it should be considered as a potential anticancer agent.
Insights
22β-hydroxytingenone (22-HTG) shows significant anti-cancer effects against human melanoma cells. This compound effectively reduced melanoma cell proliferation, migration, and invasion, indicating its potential as a novel anticancer agent.
Area of Science:
- Oncology
- Natural Products Chemistry
Background:
- Melanoma is a highly lethal skin cancer with significant invasive potential.
- Quinonemethide triterpenes are recognized for their promising anticancer properties.
Purpose of the Study:
- To investigate the anti-cancer effects of 22β-hydroxytingenone (22-HTG) on human melanoma cells.
- To evaluate the impact of 22-HTG on melanoma cell proliferation, migration, metabolism, and invasion.
Main Methods:
- Cytotoxicity was assessed using the Alamar blue assay.
- Subtoxic concentrations of 22-HTG were used to evaluate effects on cell proliferation, migration, metabolism, and invasion.
- Inhibition concentration 50 (IC50) values were determined for the SK-MEL-28 cell line.
Main Results:
- 22-HTG demonstrated cytotoxic effects with IC50 values ranging from 3.29 to 4.35 μM against SK-MEL-28 cells.
- The compound significantly reduced melanoma cell proliferation, migration, and invasion.
- 22-HTG decreased the activity of matrix metalloproteinases (MMP-2 and MMP-9) and the expression of lactate dehydrogenase (LDHA).
Conclusions:
- 22-HTG exhibits anti-proliferative and anti-invasive effects against human melanoma cells.
- The findings suggest 22-HTG is a potent substance with potential as an anticancer agent for melanoma treatment.
