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Updated: Dec 22, 2025

Analysis of Oxidative Stress in Zebrafish Embryos
Published on: July 7, 2014
[Oxidative stress in aging.]
N K Zenkov1, P M Kozhin1, A V Chechushkov1
1Federal Research Center for Fundamental and Translational Medicine, 2 Timakova str., Novosibirsk 630117, Russian Federation,
Abstract:
The free-radical theory of aging, advanced more than 50 years ago by D.Harman, remains popular today. The review analyzes age-related changes in the main endogenous mechanisms of reactive oxygen species (ROS) production and antioxidant defense mechanisms. With age, ROS generation by mitochondria, peroxisomes, and NAD(P)H oxidases is enhanced, while the transcriptional activity of the important system Keap1/Nrf2/ARE maintaining redox balance decreases. In old animals, autophagy activity is also low, which removes damaged organelles and aggregated structures from cells. The age-related shift of the redox balance towards oxidative stress can cause the development of age-associated neurodegenerative, autoimmune and inflammatory pathologies.
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