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Extracellular vesicles provide a capsid-free vector for oncolytic adenoviral DNA delivery

Heikki Saari1, Tiia Turunen2, Andres Lõhmus1

  • 1Division of Pharmaceutical Biosciences and Drug Research Program, Faculty of Pharmacy, University of Helsinki, Helsinki, Finland.

Journal of Extracellular Vesicles
|May 5, 2020
PubMed

Insights

Extracellular vesicles (EVs) carrying oncolytic adenoviruses were secreted by infected cancer cells. These infected cell-derived EVs (IEVs) can infect other cancer cells, offering potential for cancer and gene therapy.

Area of Science:

  • Oncology
  • Virology
  • Nanomedicine

Background:

  • Extracellular vesicles (EVs) are explored for therapeutic delivery, including oncolytic viruses for cancer treatment.
  • EVs may shield viruses from the immune system and facilitate cancer cell entry.

Purpose of the Study:

  • To investigate the formation and viral cargo of EVs secreted by adenovirus-infected cancer cells over time.
  • To assess the therapeutic potential of these infected cell-derived EVs (IEVs).

Main Methods:

  • Cancer cells were infected with an oncolytic adenovirus.
  • EVs secreted by infected cells (IEVs) were collected at different time points.
  • IEVs were analyzed for viral genome content and infectivity in recipient cancer cells.

Main Results:

  • IEVs were secreted before viral lysis and resembled normal EVs, not apoptotic fragments.
  • IEVs successfully carried the adenovirus genome.
  • IEVs induced infection in untreated cancer cells.

Conclusions:

  • EVs play a role in the adenovirus life cycle.
  • IEVs can be utilized for effective cancer and gene therapy delivery.
Keywords:
DNA deliveryExtracellular vesiclesadenoviruscancer therapy

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