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A simple apparatus for controlled pressure perfusion fixation.

N J Lenn, B Beebe

    Microscopica Acta
    |March 1, 1977
    PubMed
    Summary
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    This study introduces a simple, inexpensive apparatus for precise vascular perfusion in small animals. The method ensures reproducible pressure control for reliable tissue fixation, particularly for rodent brains.

    Area of Science:

    • Veterinary Medicine
    • Anatomical Pathology
    • Research Methodology

    Background:

    • Vascular perfusion is crucial for tissue fixation in small animal research.
    • Achieving consistent pressure control during perfusion can be challenging.
    • Existing methods may be complex, expensive, or lack reproducibility.

    Purpose of the Study:

    • To describe a novel, simple, and inexpensive apparatus for vascular perfusion in small animals.
    • To demonstrate quantitative and reproducible pressure control using the described apparatus.
    • To validate the efficacy of the method for reliable tissue fixation.

    Main Methods:

    • An apparatus utilizing a sphygmomanometer and a plastic blood transfer pack was developed.
    • External pressure was applied to the pack to control fixative solution delivery.

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  • Outflow pressure was directly measured to ensure pressure regulation.
  • The apparatus was tested on rodent brains across various developmental stages and other small animals.

    • Main Results:

    • The apparatus achieved quantitative and reproducible pressure control during vascular perfusion.
    • Reproducibility was maintained by using air inflation within the blood transfer pack.
    • The method demonstrated reliable and effective fixation of rodent brains and other small animal tissues.
    • The system proved simple, inexpensive, and effective for its intended purpose.

    Conclusions:

    • The described apparatus offers a reliable, cost-effective solution for vascular perfusion in small animals.
    • This method ensures consistent pressure control, leading to high-quality tissue fixation.
    • The technique is suitable for a range of small animal models, including rodents of all ages.