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Related Experiment Video

Updated: Dec 22, 2025

A New Clarification Method to Visualize Biliary Degeneration During Liver Metamorphosis in Sea Lamprey Petromyzon marinus

A New Clarification Method to Visualize Biliary Degeneration During Liver Metamorphosis in Sea Lamprey Petromyzon marinus

Published on: June 6, 2014

Fine-scale visualizing the hierarchical structure of mouse biliary tree with fluorescence microscopy method.

Yuwei Chen¹, Lin Bai², Yongjie Zhou¹

¹Laboratory of Pathology, Key Laboratory of Transplant Engineering and Immunology, NHC, West China Hospital, Sichuan University, Chengdu 610041, China.

Bioscience Reports

|May 5, 2020

Summary

Researchers developed a simple imaging method to visualize mouse liver biliary tracts in 3D. This technique clearly shows the fine-scale architecture of the biliary tree in healthy and diseased livers.

Keywords:

Biliary tract Confocal microscope Fluorescence imaging

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Area of Science:

Hepatology and Liver Disease Research
Microscopy and Imaging Techniques
Mouse Model Development

Background:

The liver's biliary tracts are crucial for function but often disrupted in liver diseases.
Understanding biliary tract structure is key to deciphering liver disease mechanisms.
Current visualization methods lack fine-scale, 3D detail.

Purpose of the Study:

To develop a simple, repeatable imaging method for visualizing mouse liver biliary tracts.
To achieve fine-scale, three-dimensional (3D) imaging of the entire biliary tree.
To observe structural differences in biliary tracts between normal and diseased liver models.

Main Methods:

Established mouse models of biliary tract injury via diet, surgery, or genetic modification.
Used fluorescent dyes (Rhodamine B, Hoechst) to label parenchymal cell cytoplasm and nuclei.
Retrogradely perfused biliary tracts with fluorescein isothiocyanate (FITC) and observed via confocal microscopy.

Main Results:

Successfully visualized the fine-scale and 3D structure of the entire mouse biliary tree.
Clearly depicted the network of end-terminal bile canaliculi and adjacent hepatocytes.
Observed distinct structural characteristics of biliary tracts in normal versus diseased liver models.

Conclusions:

A simple and repeatable imaging method was developed for visualizing liver biliary tracts.
This technique provides high-resolution, 3D insights into biliary tract architecture.
The method aids in understanding the structural basis of liver diseases affecting the biliary system.