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Long non-coding RNA SNHG7 promotes malignant melanoma progression through negative modulation of miR-9

Wendi Wang1, Guangjing Liu1, Man Liu1

  • 1Department of Plastic and Burn Surgery, Tianjin First Center Hospital, Tianjin, China.

Histology and Histopathology
|May 5, 2020
PubMed

Insights

Long non-coding small nucleolar RNA host gene 7 (SNHG7) drives malignant melanoma progression by sponging miR-9, leading to increased cell proliferation and migration. Targeting SNHG7 offers a potential therapeutic strategy for melanoma.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Long non-coding RNA SNHG7 is an oncogene in various human cancers.
  • The specific role of SNHG7 in malignant melanoma pathogenesis is not well understood.

Purpose of the Study:

  • To investigate the expression and function of SNHG7 in malignant melanoma.
  • To elucidate the molecular mechanism underlying SNHG7's role in melanoma progression.

Main Methods:

  • Analysis of SNHG7 expression in melanoma tissues and cell lines.
  • SNHG7 knockdown using siRNA.
  • Bioinformatics analysis to identify miRNA interactions.
  • Western blotting to assess protein expression levels.
  • In vivo xenograft studies.

Main Results:

  • SNHG7 expression was significantly upregulated in malignant melanoma.
  • SNHG7 knockdown inhibited melanoma cell proliferation and migration.
  • SNHG7 acted as a molecular sponge for miR-9.
  • miR-9 targeted PI3KR3, and SNHG7 knockdown led to decreased expression of PI3KR3, pAKT, cyclin D1, and Girdin.
  • SNHG7 knockdown suppressed tumor growth in vivo.

Conclusions:

  • SNHG7 functions as an oncogene in malignant melanoma.
  • The SNHG7/miR-9/PI3KR3 axis plays a critical role in melanoma development.
  • SNHG7 represents a potential therapeutic target for malignant melanoma.

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