Related Experiment Video
Updated: Dec 22, 2025
10:24
Two- and Three-Dimensional Live Cell Imaging of DNA Damage Response Proteins
Published on: September 28, 2012
14.5K
The Licensing Factor Cdt1 Links Cell Cycle Progression to the DNA Damage Response
Alexandra Kanellou1, Nickolaos Nikiforos Giakoumakis1, Andreas Panagopoulos1
1School of Medicine, University of Patras, Patras, Greece.
Anticancer Research
|May 6, 2020
Summary
Chromatin licensing and DNA replication factor 1 (Cdt1) ensures DNA replication occurs only once per cell cycle. Cdt1 also aids in DNA damage repair, maintaining genome stability by linking cell cycle control and DNA damage responses.
Area of Science:
- Molecular Biology
- Cell Biology
- Genetics
Background:
- Genome integrity is crucial for cell survival, requiring precise DNA replication and repair.
- Genomic instability, arising from errors in DNA replication or unrepaired lesions, is linked to cancer development.
- Chromatin licensing and DNA replication factor 1 (Cdt1) regulates DNA replication timing.
Purpose of the Study:
- To elucidate the cellular functions of Cdt1.
- To explore the role of Cdt1 in interlinking cell cycle regulation and DNA damage response pathways.
- To understand Cdt1's contribution to maintaining genome stability.
Main Methods:
- Discusses the known functions of Cdt1 in DNA replication licensing.
- Reviews Cdt1's involvement in DNA damage response.
- Examines the mechanism of Cdt1 poly-ubiquitination and degradation by CRL4^Cdt2 upon DNA damage.
Main Results:
- Cdt1 exclusively loads the minichromosome maintenance 2-7 helicase complex onto chromatin during the G1 phase, restricting DNA replication to once per cell cycle.
- Following DNA damage, Cdt1 accumulates at damage sites and undergoes poly-ubiquitination mediated by the CRL4^Cdt2 complex, leading to its degradation.
- These findings highlight Cdt1's dual role in replication control and DNA damage management.
Conclusions:
- Cdt1 plays a critical role in preventing re-replication and maintaining genome stability.
- Cdt1 acts as a molecular link between cell cycle progression and DNA damage surveillance.
- Understanding Cdt1's functions provides insights into mechanisms preventing genomic instability and cancer.
More Related Videos
08:33Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis
Published on: December 5, 2017
14.8K
08:21Cell Cycle-specific Measurement of γH2AX and Apoptosis After Genotoxic Stress by Flow Cytometry
Published on: September 1, 2019
14.0K
Related Concept Videos
02:36DNA Damage can Stall the Cell Cycle
9.9K
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
9.9K
02:36DNA Damage Can Stall the Cell Cycle
2.9K
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
2.9K
02:34Inhibition of Cdk Activity
5.5K
The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
5.5K
01:28The Cell Cycle Control System
5.1K
The cell cycle regulation directs how a cell proceeds from one phase to the next and begins mitosis. The cell cycle control system includes intracellular regulatory molecules and external triggers. They provide "stop" or "advance" signals and operate at specific cell cycle stages termed checkpoints to ensure that a particular process is completed before the cell advances to the next phase.
Cyclins and cyclin-dependent kinases (Cdks) are the primary cell cycle regulators and...
Cyclins and cyclin-dependent kinases (Cdks) are the primary cell cycle regulators and...
5.1K
02:11The Cell Cycle Control System
13.7K
The cell cycle is an organized set of events that leads the cell to divide into two daughter cells, each containing chromosomes identical to the parent cell. It is the cell cycle that leads to the formation of an entire organism from a single-cell zygote. Besides, cell division also functions in the renewal or repair of tissues in adult multicellular eukaryotes. For example, in the bone marrow, the stem cells divide to form new blood cells. Although essential for several functions, cell...
13.7K
02:39Positive Regulator Molecules
6.4K
Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.
6.4K