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The Plant Infection Test: Spray and Wound-Mediated Inoculation with the Plant Pathogen Magnaporthe Grisea
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Ferroptosis contributes to developmental cell death in rice blast
Qing Shen1, Meiling Liang2, Fan Yang1
1Temasek Life Sciences Laboratory, and Department of Biological Sciences, National University of Singapore, 1 Research Link, Singapore, 117604, Republic of Singapore.
The New Phytologist
|May 6, 2020
Summary
Ferroptosis, an iron-dependent cell death, is crucial for the rice blast fungus Magnaporthe oryzae's development and infection. Inhibiting ferroptosis hinders fungal invasion, highlighting its role in pathogenesis.
Area of Science:
- Molecular Biology
- Mycology
- Plant Pathology
Background:
- Ferroptosis is an iron-dependent form of regulated cell death.
- Magnaporthe oryzae causes rice blast, a devastating plant disease.
Purpose of the Study:
- To investigate the occurrence and role of ferroptosis in Magnaporthe oryzae.
- To elucidate the regulatory mechanisms and functional significance of ferroptosis in fungal pathogenesis.
Main Methods:
- Confirmation of ferroptosis using five criteria.
- Optimized ratio-fluorescence imaging with C11-BODIPY581/591 for lipid peroxides.
- Assessed roles of reduced glutathione and NADPH oxidases.
- Investigated ferroptosis inhibition via iron chelation and chemical agents.
- Examined ferroptosis and autophagy interplay.
Main Results:
- Ferroptosis occurs in M. oryzae and is dependent on ferric ions.
- Reduced glutathione and NADPH oxidases regulate ferroptotic cell death.
- Ferroptosis is essential for conidial developmental cell death during appressorium maturation.
- Inhibition of ferroptosis impairs M. oryzae host invasion and growth.
- Ferroptosis and autophagy have interdependent roles in cell death regulation.
Conclusions:
- Ferroptosis is a key process in M. oryzae development and pathogenesis.
- Understanding ferroptosis offers insights into fungal iron homeostasis and disease control strategies.
- Targeting ferroptosis could be a novel approach to manage rice blast disease.
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