Related Experiment Video

Updated: Dec 22, 2025

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis
11:44

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

7.9K

LINC00641 hinders the progression of cervical cancer by targeting miR-378a-3p/CPEB3

Yan Zhang1, Rong Yu2, Lei Li1

  • 1Department of Obstetrics, the Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, Shandong, China.

The Journal of Gene Medicine
|May 6, 2020
PubMed
Abstract

Insights

Long non-coding RNA LINC00641 suppresses cervical cancer (CC) progression by targeting the miR-378a-3p/CPEB3 pathway. This suggests LINC00641 holds therapeutic potential for CC treatment.

Area of Science:

  • Oncology
  • Molecular Biology
  • Gene Regulation

Background:

  • Long non-coding RNA LINC00641 exhibits anti-tumor properties across various cancers.
  • Its specific role in cervical cancer (CC) remains largely undetermined.

Purpose of the Study:

  • To elucidate the functional role and underlying molecular mechanisms of LINC00641 in cervical cancer.
  • To investigate the potential of LINC00641 as a therapeutic target for CC.

Main Methods:

  • Quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) to assess expression levels of LINC00641, miR-378a-3p, and CPEB3.
  • RNA pull-down and luciferase reporter assays to determine the regulatory interactions between LINC00641 and its downstream targets.
  • In vitro experiments to evaluate the effects of LINC00641 manipulation on CC cell proliferation, migration, invasion, apoptosis, and epithelial-mesenchymal transition (EMT).

Main Results:

  • LINC00641 was significantly under-expressed in CC cell lines.
  • Overexpression of LINC00641 inhibited CC cell proliferation, migration, invasion, and EMT, while increasing apoptosis.
  • LINC00641 directly targeted miR-378a-3p, leading to its downregulation, and miR-378a-3p was upregulated in CC.
  • CPEB3 was identified as a downstream target of miR-378a-3p and was under-expressed in CC; knockdown of CPEB3 partially reversed the effects of LINC00641 overexpression.

Conclusions:

  • LINC00641 suppresses cervical cancer progression by negatively regulating the miR-378a-3p/CPEB3 axis.
  • LINC00641 demonstrates potential as a novel therapeutic agent for cervical cancer treatment.
Keywords:
CPEB3LINC00641cervical cancermiR-378a-3p

More Related Videos

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice07:02

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice

Published on: August 23, 2019

7.7K
Quantitative PCR-based Assay to Measure Sonic Hedgehog Signaling in Cellular Model of Ciliogenesis07:26

Quantitative PCR-based Assay to Measure Sonic Hedgehog Signaling in Cellular Model of Ciliogenesis

Published on: January 31, 2025

930

Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.6K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
23.7K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
5.0K
Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
5.5K
Induced Pluripotent Stem Cells01:06

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic...
5.2K
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
9.6K