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Updated: Dec 22, 2025

Monitoring eIF4F Assembly by Measuring eIF4E-eIF4G Interaction in Live Cells
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Roles of eIF3m in the tumorigenesis of triple negative breast cancer

Wei Han1, Cong Zhang2, Chun-Tao Shi3

  • 11Department of General Surgery, Kunshan First People's Hospital Affiliated to Jiangsu University, Kunshan Jiangsu, 215300 People's Republic of China.

Cancer Cell International
|May 6, 2020
PubMed
Abstract

Insights

Eukaryotic translation initiation factor 3m (eIF3m) promotes triple-negative breast cancer (TNBC) progression. Lowering eIF3m levels suppresses TNBC cell growth, migration, and invasion, indicating its role as an unfavorable prognostic marker.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Research

Background:

  • Triple-negative breast cancer (TNBC) presents a poor prognosis due to a lack of targeted therapies.
  • Eukaryotic translation initiation factor 3m (eIF3m) is implicated in the malignant progression of various cancers.

Purpose of the Study:

  • To investigate the biological functions and prognostic significance of eIF3m in TNBC.
  • To explore the role of eIF3m in TNBC cell proliferation, migration, invasion, and apoptosis.

Main Methods:

  • Bioinformatic analysis using Oncomine and KM-plotter databases for prognosis.
  • In vitro assays including CCK-8, colony formation, cell cycle, wound healing, Transwell, mitochondrial membrane potential, and apoptosis assays after eIF3m knockdown.
  • Western blotting to elucidate molecular mechanisms and identify regulated genes.

Main Results:

  • Elevated eIF3m expression correlates with poorer survival outcomes in breast cancer patients.
  • eIF3m is significantly upregulated in TNBC tissues and associated with advanced disease features.
  • Knockdown of eIF3m suppressed TNBC cell proliferation, migration, invasion, and mitochondrial potential, while increasing apoptosis.
  • eIF3m influences cell cycle, epithelial-mesenchymal transition, and apoptosis-related proteins, modulating 14 key genes in TNBC development.

Conclusions:

  • eIF3m serves as an unfavorable prognostic indicator for TNBC.
  • eIF3m plays a critical role in TNBC tumorigenesis and progression.
Keywords:
BioinformationTriple negative breast cancerTumorigenesiseIF3m

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