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Lactoferrin quantification in cattle faeces by ELISA.

Andrew S Cooke1,2, Kathryn A Watt3, Greg F Albery3

  • 1Rothamsted Research, North Wyke, Okehampton, UK.

Peerj
|May 6, 2020
PubMed
Summary
This summary is machine-generated.

Quantifying lactoferrin in cattle faeces using ELISA is feasible, offering a non-invasive method for assessing animal health. This approach avoids blood sampling and shows potential for monitoring bovine immunology and pathology.

Keywords:
AgricultureAnimalsEcologyImmunologyLivestockOne-healthVeterinary medicineVeterinary scienceZoology

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Area of Science:

  • Veterinary immunology
  • Ruminant health management
  • Biomarker discovery

Background:

  • Maintaining ruminant health is crucial for animal welfare and productivity.
  • Faecal lactoferrin measurement in humans aids gastrointestinal illness assessment without blood sampling.
  • This non-invasive approach may be applicable to cattle health monitoring.

Purpose of the Study:

  • To evaluate the efficacy of a bovine lactoferrin enzyme-linked immunosorbent assay (ELISA) for quantifying lactoferrin in cattle faeces.
  • To compare faecal lactoferrin concentrations with background levels.
  • To assess the correlation between faecal and serum lactoferrin levels.

Main Methods:

  • An established bovine lactoferrin ELISA (for serum and milk) was adapted for faecal supernatant analysis.
  • Faecal samples were analyzed for lactoferrin concentration and compared to background readings.
  • Faecal and serum lactoferrin levels from the same animals were compared.

Main Results:

  • Faecal samples showed significantly higher optical densities than background, confirming assay effectiveness.
  • Mean faecal lactoferrin concentration was 0.269 µg mL⁻¹ (S.E. 0.031).
  • Faecal and serum lactoferrin concentrations differed significantly (p=0.039) and did not correlate (r=0.2699, p=0.238).

Conclusions:

  • Lactoferrin can be successfully quantified in cattle faeces using ELISA.
  • This method holds potential for non-invasive assessment of cattle immunology and pathology.
  • Further research is needed to identify the physiological source of faecal lactoferrin.