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Updated: Dec 22, 2025
A Tuberculosis Molecular Bacterial Load Assay TB-MBLA
Published on: April 30, 2020
Improving sputum collection processes to increase tuberculosis case finding among HIV-positive persons in Botswana
U Mathebula1, C Emerson2, T Agizew1
1Centers for Disease Control and Prevention (CDC), Gaborone, Botswana.
Setting:
Twenty-two human immunodeficiency virus (HIV) clinics in Botswana.
Objective:
To compare sputum collection rates, sputum quality and volume, and tuberculosis (TB) diagnosis rates before and after field efforts to improve sputum collection among individuals newly diagnosed with HIV with TB symptoms.
Design:
Newly diagnosed individuals living with HIV attending 22 HIV clinics in Botswana were screened for TB from August 2012 to March 2014. Starting in May 2013, a field intervention composed of the introduction of a tracking log for presumed TB patients, and patient instructions and sputum induction to improve sputum collection rates was implemented.
Results:
Prior to the intervention, sputum collection rates were 44.1% (384/870). Subsequently, sputum collection increased to 58.3% (579/993) (P < 0.001). Sputum quality and volume also improved. Although rates of TB diagnosis increased from 9.7% (84/870) to 12.5% (120/993), this difference was not significant (P = 0.143).
Conclusion:
Sputum collection rates among presumptive TB cases, as well as sputum quality and volume improved after implementation of the field intervention. To improve sputum collection rates, efforts at the program level should be ongoing.
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