Related Experiment Video
Updated: Dec 22, 2025

Analysis of Group IV Viral SSHHPS Using In Vitro and In Silico Methods
Published on: December 21, 2019
Leafhopper Psammotettix alienus hosts chuviruses with different genomic structures
1State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Abstract:
The leafhopper Psammotettix alienus causes serious economic losses by directly sucking the plant sap and by transmitting plant viruses. Here, we obtained the full-genomic sequence of a novel chuvirus, Hancheng leafhopper mivirus (HCLeV), using RNA sequencing (RNA-seq) and rapid amplification of cDNA ends (RACE). The full genome of HCLeV comprises 9,852 nucleotides (nt) and includes two open reading frames (ORFs), which encode nucleocapsid and large polymerase protein, respectively. Its genomic features are similar to that of Tacheng tick virus 5, a member of chuviruses. However, HCLeV had only 36.6-44.4% identities in amino acid sequence for polymerase and 27.1-44.2% identities for nucleocapsid protein with chuviruses and other viruses in the order Mononegavirales and Jingchuvirales. Interestingly, we found this leafhopper can host two chuvirus species with different genomic structures. The discovery of this new virus potentially adds a new species to the family Chuviridae, and its new genomic structural form indicates broader genomic diversity among the chuviruses.
More Related Videos
11:12Protocols for Investigating the Host-tissue Distribution, Transmission-mode, and Effect on the Host Fitness of a Densovirus in the Cotton Bollworm
Published on: April 12, 2017
06:02Arbovirus Infections As Screening Tools for the Identification of Viral Immunomodulators and Host Antiviral Factors
Published on: September 13, 2018
Related Concept Videos
Viruses of Archaea
Subviral Agents
Diversity of Protists I
Bacterial Phylum Chlamydiae
Size and Structure of Viral Genomes
Viral Recombination