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High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry

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Trends using biological target-based assays for drug detection in complex sample matrices.

Michael F Santillo¹

¹Division of Toxicology, Office of Applied Research and Safety Assessment, Center for Food Safety and Applied Nutrition, U.S. Food and Drug Administration (FDA), 8301 Muirkirk Rd, Laurel, MD, 20708, USA. michael.santillo@fda.hhs.gov.

Analytical and Bioanalytical Chemistry

|May 7, 2020

Summary

Drug biosensors utilizing biological targets can detect drug classes without knowing their structure. These assays are advancing for detecting illicit drugs in supplements, food animals, and bodily fluids.

Keywords:

Bioassays Biosensors Drug monitoring/drug screening Enzymes Pharmaceuticals Receptors/ion channels

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Area of Science:

Pharmacology
Analytical Chemistry
Biotechnology

Background:

Drug molecules interact with biological targets in vivo, producing therapeutic effects.
Drug biosensors, based on drug-target interactions, can detect entire drug classes without prior structural knowledge.
Current analytical methods often require specific knowledge of drug structures, limiting broad detection.

Purpose of the Study:

To review recent advancements in bio-target-based screening assays for drug detection.
To highlight applications in detecting drugs in illicit supplements, food-producing animals, and bodily fluids.
To discuss challenges and future directions for bio-target drug detection assays.

Main Methods:

Review of recent scientific literature on bio-target-based drug detection assays.
Focus on assays utilizing enzymes, receptors, ion channels, and transporters as biosensors.
Analysis of assay applicability in diverse matrices like supplements, animal products, and biological samples.

Main Results:

Bio-target assays show promise for broad-spectrum drug detection across various classes.
Recent developments enable sensitive detection of drugs in complex matrices.
These assays offer a complementary approach to traditional analytical techniques.

Conclusions:

Bio-target-based drug biosensors represent a significant advancement in drug detection capabilities.
Future applications are anticipated for enhanced screening of illicit products and in clinical diagnostics.
Overcoming current challenges will further expand the utility of these powerful detection tools.