Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Overview of Cell-Matrix Interactions01:24

Overview of Cell-Matrix Interactions

8.7K
The extracellular matrix or ECM holds cells together to form a tissue and allows the cells within the tissue to communicate. ECM comprises proteins such as fibronectin, collagen, laminin, etc. The most abundant protein in this space is collagen. Collagen fibers are interwoven with carbohydrate-containing protein molecules called proteoglycans. ECM allows cell migration and provides a structural scaffold at cell adhesion that anchors the cell when the extracellular matrix proteins interact with...
8.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Risk factors for acquisition of SARS-CoV-2 Omicron variant among a prospective cohort of French Healthcare Workers: impact of hybrid immunity.

Scientific reports·2026
Same author

Conditioned accumbal dopamine transients forecast individual preference for drug versus natural rewards and compulsive behavior.

Nature neuroscience·2026
Same author

Clinic-to-Mechanism: Unraveling in-depth molecular dysfunctions caused by a GluN2B C-Terminal deletion in developmental and epileptic encephalopathies.

Translational psychiatry·2026
Same author

From eye to AI: Studying rodent social behavior in the era of machine learning.

Neuroscience and biobehavioral reviews·2026
Same author

The sodium leak channel NALCN in Drd2<sup>+</sup> striatal neurons regulates neuronal excitability, locomotion and food-seeking in a sex-dependent manner.

Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology·2026
Same author

Daily intermittent fasting is an effective multiscale treatment in preclinical models of absence epilepsy.

Epilepsia·2025

Related Experiment Video

Updated: Dec 22, 2025

A Model of Epileptogenesis in Rhinal Cortex-Hippocampus Organotypic Slice Cultures
10:05

A Model of Epileptogenesis in Rhinal Cortex-Hippocampus Organotypic Slice Cultures

Published on: March 18, 2021

7.4K

Gelatinase Biosensor Reports Cellular Remodeling During Epileptogenesis.

Nathalie Bouquier1, Benoit Girard1, Juri Aparicio Arias1

  • 1IGF, Université de Montpellier, CNRS, INSERM, Montpellier, France.

Frontiers in Synaptic Neuroscience
|May 7, 2020
PubMed
Summary

A novel biosensor detects gelatinase activity, crucial for epileptogenesis (the process of developing epilepsy). This tool visualizes enzyme activity in real-time, aiding in understanding and potentially treating epilepsy.

Keywords:
activatable cell-penetrating peptides (ACPPs)epileptogenesisgelatinasekainate (KA)matrix metalloproteinases (MMPs)molecular imaging probes

More Related Videos

In Vivo Fiber-Coupled Pre-Clinical Confocal Laser-scanning Endomicroscopy pCLE of Hippocampal Capillaries in Awake Mice
09:08

In Vivo Fiber-Coupled Pre-Clinical Confocal Laser-scanning Endomicroscopy pCLE of Hippocampal Capillaries in Awake Mice

Published on: April 21, 2023

1.2K
Electroconvulsive Seizures in Rats and Fractionation of Their Hippocampi to Examine Seizure-induced Changes in Postsynaptic Density Proteins
09:07

Electroconvulsive Seizures in Rats and Fractionation of Their Hippocampi to Examine Seizure-induced Changes in Postsynaptic Density Proteins

Published on: August 15, 2017

12.5K

Related Experiment Videos

Last Updated: Dec 22, 2025

A Model of Epileptogenesis in Rhinal Cortex-Hippocampus Organotypic Slice Cultures
10:05

A Model of Epileptogenesis in Rhinal Cortex-Hippocampus Organotypic Slice Cultures

Published on: March 18, 2021

7.4K
In Vivo Fiber-Coupled Pre-Clinical Confocal Laser-scanning Endomicroscopy pCLE of Hippocampal Capillaries in Awake Mice
09:08

In Vivo Fiber-Coupled Pre-Clinical Confocal Laser-scanning Endomicroscopy pCLE of Hippocampal Capillaries in Awake Mice

Published on: April 21, 2023

1.2K
Electroconvulsive Seizures in Rats and Fractionation of Their Hippocampi to Examine Seizure-induced Changes in Postsynaptic Density Proteins
09:07

Electroconvulsive Seizures in Rats and Fractionation of Their Hippocampi to Examine Seizure-induced Changes in Postsynaptic Density Proteins

Published on: August 15, 2017

12.5K

Area of Science:

  • Neuroscience
  • Biochemistry
  • Molecular Imaging

Background:

  • Epileptogenesis transforms a healthy brain into an epileptic one, involving synaptic plasticity, neuroinflammation, and cell death.
  • Matrix Metalloproteinases (MMPs), particularly gelatinases (MMP-2 and MMP-9), are implicated in extracellular matrix remodeling and epileptogenesis.

Purpose of the Study:

  • To engineer and validate a biosensor for in situ reporting of gelatinase activity in an epilepsy model.
  • To investigate the spatiotemporal distribution of gelatinase activity during epileptogenesis.

Main Methods:

  • Development of a gelatinase-sensitive activatable cell penetrating peptide (ACPP) coupled to a TAMRA fluorophore.
  • Utilizing a preclinical mouse model of temporal lobe epilepsy (TLE) induced by intrahippocampal kainate injection.
  • Employing the ACPP biosensor for fluorescence-based detection of gelatinase activity.

Main Results:

  • The ACPP biosensor successfully reported localized gelatinase activities in the TLE mouse model.
  • Gelatinase activity was observed in the ipsilateral hippocampus, originating in the CA1 area and spreading to the dentate gyrus.
  • Activity was detected in neurons and microglial cells from early stages through chronic epilepsy.

Conclusions:

  • ACPPs serve as effective molecular imaging probes for detecting gelatinase activity patterns during epileptogenesis.
  • The findings highlight the spatiotemporal dynamics of gelatinase activity in epilepsy.
  • ACPPs may offer potential as vectors for targeted treatment delivery in epileptogenesis.