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Inhibition of long non-coding RNA TSIX accelerates tibia fraction healing via binding and positively regulating the
1Department of Orthopedics Trauma, Trauma Center, Shanghai General Hospital of Nanjing Medical University, Shanghai, China. wangqiugen@yahoo.com.
Summary
Knocking down long chain non-coding RNA TSIX (lncRNA TSIX) promotes tibia fracture healing by inhibiting SOX6 expression. This suggests lncRNA TSIX/SOX6 may be a therapeutic target for fracture treatment.
Area of Science:
- Molecular Biology
- Genetics
- Orthopedics
Background:
- Fracture healing is a complex process influenced by gene expression.
- Long chain non-coding RNAs (lncRNAs) are increasingly recognized for their roles in biological processes.
- Tibial fractures (TF) require long-term treatment and have a slow recovery.
Purpose of the Study:
- To investigate the role of lncRNA TSIX in tibial fracture healing.
- To explore the molecular mechanism of lncRNA TSIX in fracture repair.
- To identify potential therapeutic targets for TF.
Main Methods:
- Tibial fracture models were established in male C57BL/6J mice.
- Osteoblast cell cultures were used as an in vitro model.
- Expression of lncRNA TSIX and osteogenesis-related genes (Col1a1, Col-II, Col-X) was analyzed.
- Proliferation and apoptosis were assessed.
- Hematoxylin-eosin (HE) staining was performed to evaluate fracture healing.
Main Results:
- lncRNA TSIX expression was upregulated in plasma of TF mice in a time-dependent manner.
- Overexpression of lncRNA TSIX inhibited osteoblast proliferation and promoted apoptosis.
- lncRNA TSIX positively regulated SOX6 expression, affecting osteogenesis-related genes.
- Knockdown of lncRNA TSIX improved fracture healing in vivo and in vitro.
Conclusions:
- lncRNA TSIX knockdown promotes tibia fracture healing by inhibiting SOX6 expression.
- The lncRNA TSIX/SOX6 axis is a potential therapeutic target for tibial fracture treatment.
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