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MicroRNA-493-5p suppresses colorectal cancer progression via the PI3K-Akt-FoxO3a signaling pathway

F-C Cui1, Y Chen, X-Y Wu

  • 1Department of Clinical Laboratory, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, Zhengzhou, Henan, Jinshui District, Zhengzhou City, Henan, P.R. China. facaichn@163.com.

European Review for Medical and Pharmacological Sciences
|May 7, 2020
PubMed
Abstract

Insights

MicroRNA493-5p (miR-493-5p) suppresses colorectal cancer (CRC) progression by inhibiting the PI3K-Akt-FoxO3a pathway. This microRNA is downregulated in CRC and its restoration reduces cancer cell proliferation, migration, and invasion.

Area of Science:

  • Molecular Biology
  • Oncology
  • Gene Regulation

Background:

  • MicroRNA493-5p (miR-493-5p) is implicated in cancer cell proliferation and migration.
  • The specific role and mechanism of miR-493-5p in colorectal cancer (CRC) remain largely unelucidated.

Purpose of the Study:

  • To investigate the function and mechanism of miR-493-5p in colorectal cancer (CRC) progression.
  • To determine the molecular targets and signaling pathways regulated by miR-493-5p in CRC.

Main Methods:

  • Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR) for miR-493-5p expression analysis in CRC tissues and cell lines.
  • Cell proliferation, migration, and invasion assays (colony formation, transwell, wound-healing) following miR-493-5p mimic transfection or PI3K agonist treatment.
  • Luciferase reporter assays, Western blotting, and immunofluorescence to assess the miR-493-5p/PI3K interaction and downstream signaling (PI3K, Akt, FoxO3a).

Main Results:

  • MiR-493-5p expression was significantly downregulated in CRC tissues and cell lines, correlating with disease progression.
  • Overexpression of miR-493-5p inhibited CRC cell proliferation, migration, and invasion.
  • MiR-493-5p directly targets PI3K, leading to decreased expression of PI3K, Akt, and FoxO3a proteins. PI3K agonist treatment counteracted the suppressive effects of miR-493-5p.

Conclusions:

  • MiR-493-5p acts as a tumor suppressor in colorectal cancer.
  • The miR-493-5p-mediated suppression of CRC progression occurs through inhibition of the PI3K-Akt-FoxO3a signaling pathway.

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