Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Variables Affecting Phosphorescence and Fluorescence01:26

Variables Affecting Phosphorescence and Fluorescence

1.1K
Fluorescence and phosphorescence are essential phenomena in fields like analytical chemistry, biological imaging, and materials science, where they detect molecular properties and visualize cellular structures. Understanding the variables that influence these luminescent behaviors is crucial for maximizing accuracy and efficiency in their applications. These variables can broadly be grouped into chemical structure, solvent properties, and external conditions, each playing a distinct role in...
1.1K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

12.1K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
12.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Design and analysis of modified octagonal ring-shaped MIMO antenna with connected ground for 5G sub-6 GHz n79, Wi-Fi 5, and Wi-Fi 6E band applications.

Scientific reports·2026
Same author

Reconfigurable MIMO antenna for Sub-6 GHz 5G cognitive radio with machine learning based metaheuristic optimization.

Scientific reports·2026
Same author

Metasurface loaded multi-port tunable silicon-graphene based THz antenna with LHCP/RHCP characteristics, low-RCS and low-coupling features.

Scientific reports·2026
Same author

Patterns and associated factors of complementary and alternative therapy use for dysmenorrhea among undergraduate students in North India.

AJOG global reports·2026
Same author

A dominant role of cell death in limiting Chandipura virus propagation at cell-saturating high multiplicity of infection.

mBio·2026
Same author

Ethnophytotoxicological assessment of toxic flora in Samba District, J&K: The 'first' voucher-linked baseline study.

Toxicon : official journal of the International Society on Toxinology·2026

Related Experiment Video

Updated: Dec 22, 2025

Fluorescence-quenching of a Liposomal-encapsulated Near-infrared Fluorophore as a Tool for In Vivo Optical Imaging
10:55

Fluorescence-quenching of a Liposomal-encapsulated Near-infrared Fluorophore as a Tool for In Vivo Optical Imaging

Published on: January 5, 2015

16.6K

Fluorescence Quenching by Nitro Compounds within a Hydrophobic Deep Eutectic Solvent.

Divya Dhingra1, Bhawna1, Ashish Pandey1

  • 1Department of Chemistry, Indian Institute of Technology Delhi, Hauz Khas, New Delhi 110016, India.

The Journal of Physical Chemistry. B
|May 7, 2020
PubMed
Summary
This summary is machine-generated.

Hydrophobic deep eutectic solvents (DESs) offer eco-friendly alternatives to organic solvents. Pyrene fluorescence quenching effectively characterizes these DESs and their interactions with nitro compounds.

More Related Videos

Fluorescence detection methods for microfluidic droplet platforms
14:16

Fluorescence detection methods for microfluidic droplet platforms

Published on: December 10, 2011

22.7K
Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules
10:57

Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules

Published on: November 2, 2009

13.2K

Related Experiment Videos

Last Updated: Dec 22, 2025

Fluorescence-quenching of a Liposomal-encapsulated Near-infrared Fluorophore as a Tool for In Vivo Optical Imaging
10:55

Fluorescence-quenching of a Liposomal-encapsulated Near-infrared Fluorophore as a Tool for In Vivo Optical Imaging

Published on: January 5, 2015

16.6K
Fluorescence detection methods for microfluidic droplet platforms
14:16

Fluorescence detection methods for microfluidic droplet platforms

Published on: December 10, 2011

22.7K
Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules
10:57

Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules

Published on: November 2, 2009

13.2K

Area of Science:

  • Physical Chemistry
  • Materials Science

Background:

  • Hydrophobic deep eutectic solvents (DESs) are emerging as sustainable alternatives to traditional organic solvents and ionic liquids.
  • Their unique properties make them promising for various chemical applications, including sensing and separation.

Purpose of the Study:

  • To investigate the structural features and solute dynamics of a prototypical hydrophobic DES, TBAC-DA.
  • To evaluate the potential of pyrene fluorescence quenching as a tool for characterizing DESs and detecting nitro compounds.

Main Methods:

  • Utilized pyrene fluorescence spectroscopy to study a hydrophobic DES formed by tetra-n-butylammonium chloride (TBAC) and n-decanoic acid (DA).
  • Examined the temperature-dependent quenching of pyrene fluorescence by five different nitro compounds.
  • Analyzed fluorescence emission intensity, polarity, and excited-state decay dynamics.

Main Results:

  • All five nitro compounds dynamically quenched pyrene fluorescence in TBAC-DA, following Stern-Volmer kinetics.
  • Quenching efficiency varied, with nitromethane showing higher quenching in TBAC-DA compared to hydrophilic DESs and ionic liquids.
  • Diffusion behavior was found to be dependent on quencher structure, with individual pyrene-quencher pairs conforming to the Stokes-Einstein relation.

Conclusions:

  • Pyrene fluorescence is a robust method for characterizing hydrophobic DESs and their interactions.
  • Hydrophobic DESs like TBAC-DA can serve as effective media for the detection and assessment of nitro compounds.