Related Experiment Video

Updated: Dec 22, 2025

Exosomal miRNA Analysis in Non-small Cell Lung Cancer NSCLC Patients' Plasma Through qPCR: A Feasible Liquid Biopsy Tool
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Exosomal miRNA Analysis in Non-small Cell Lung Cancer NSCLC Patients' Plasma Through qPCR: A Feasible Liquid Biopsy Tool

Published on: May 27, 2016

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Exosomal long non-coding RNA MSTRG.292666.16 is associated with osimertinib (AZD9291) resistance in non-small cell

Qinfang Deng1, Qiyu Fang1, Boxiong Xie2

  • 1Department of Oncology, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, China.

Aging
|May 7, 2020
PubMed

Insights

Exosomes play a role in osimertinib resistance in non-small cell lung cancer (NSCLC). Exosomal lncRNA MSTRG.292666.16 was found to be upregulated and contribute to this resistance.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Acquired resistance to osimertinib is a significant clinical challenge in non-small cell lung cancer (NSCLC) treatment.
  • The precise molecular mechanisms underlying osimertinib resistance remain incompletely understood.

Purpose of the Study:

  • To investigate the role of exosomes in mediating osimertinib resistance in NSCLC.
  • To identify specific molecular players, such as long non-coding RNAs (lncRNAs), involved in exosome-mediated resistance.

Main Methods:

  • Isolation and characterization of exosomes from NSCLC cells and plasma.
  • LncRNA sequencing to identify differentially expressed lncRNAs between sensitive and resistant samples.
  • Functional studies involving exosome uptake, gene expression analysis, and knockdown experiments.

Main Results:

  • LncRNA sequencing identified 123 differentially expressed lncRNAs.
  • LncRNA MSTRG.292666.16 was significantly upregulated in osimertinib-resistant cells, plasma, and their derived exosomes.
  • Osimertinib-resistant exosomes were taken up by sensitive cells, inducing resistance and altering gene expression (miRNA-21, miRNA-125b, TGFβ, ARF6, c-Kit).
  • Knockdown of lncRNA MSTRG.292666.16 reduced osimertinib resistance in H1975R cells.

Conclusions:

  • Exosomal lncRNA MSTRG.292666.16 is implicated in the development of osimertinib resistance in NSCLC.
  • This lncRNA may serve as a potential therapeutic target or biomarker for overcoming treatment resistance.
Keywords:
epidermal growth factor receptorexosomeslong non-coding RNAsnon-small cell lung cancerosimertinib resistance

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